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Specifications:
| Application | Cell culture |
| Storage Temperature | –80°C |
| Product Type | cell Lines |
| Product Brand | Thermo Fisher Scientific™, Invitrogen |
| Product Grade | Cell Culture |
Engineered HEK293 Cell Line for Rapid Generation of Stable, High-Expressing Clones
The Invitrogen™ Flp-In™-293 cell line is a human embryonic kidney (HEK293)–derived cell line engineered to contain a single Flp Recombination Target (FRT) site stably integrated at a transcriptionally active genomic locus. Designed for use with Flp-In™ expression vectors, this system enables rapid and efficient generation of stable cell lines that exhibit consistent and high-level expression of a gene of interest.
Flp-In™-293 cells were developed by transfecting parental 293 cells with pFRT/lacZeo, followed by selection of Zeocin™-resistant clones. The engineered FRT site enables targeted integration of your gene of interest via Flp recombinase-mediated recombination, significantly simplifying stable clone generation and ensuring reproducibility across experiments.
Key Features and Benefits
-
Site-Specific Integration
Contains a single, genomically integrated FRT site that enables recombinase-mediated gene insertion at a defined locus. -
High and Consistent Expression
Transgenes integrated via the Flp-In™ system are expressed at high levels from transcriptionally active chromatin, driven by CMV promoters. -
Streamlined Workflow
Rapid and reproducible generation of isogenic stable cell lines, reducing variability in expression and enabling faster data collection. -
Fully Compatible
Optimized for use with Flp-In™ vectors such as pcDNA™5/FRT, pcDNA5/FRT/V5-His-TOPO™, and pSecTag/FRT/V5-His-TOPO™. -
Zeocin™ Resistance Marker
Selection of correctly engineered cells is simplified using Zeocin™ resistance.
Applications
- Stable expression of recombinant proteins in human cells
- Functional genomics studies requiring controlled gene integration
- Target validation and drug screening
- Production of secreted or membrane-bound proteins
- High-throughput protein expression for assay development
Technical Specifications
| Property | Details |
|---|---|
| Product Name | Flp-In™-293 Cell Line |
| Catalog Number | R75007 |
| Cell Line Type | HEK293-derived, engineered with FRT site |
| Species Origin | Human |
| Resistance Marker | Zeocin™ |
| Promoter Compatibility | CMV promoter (cytomegalovirus) |
| Compatible Vectors | pcDNA™5/FRT, pcDNA™5/FRT/V5-His-TOPO™, pSecTag/FRT/V5-His-TOPO™ |
| Cell Format | Frozen cells (1 × 10⁷ cells/mL) |
| Supplied Medium | 90% complete growth medium + 10% DMSO |
| Quantity | 1 mL |
| Storage Conditions | Store in liquid nitrogen (–150°C or below) |
| Shipping Conditions | Shipped on dry ice |
| Shelf Life | 6 months from date of receipt (if stored properly in liquid nitrogen) |
| Product Line | Flp-In™ |
| Unit Size | Each |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Invitrogen™ Flp-In™-293 Cell Line provides a powerful and reproducible platform for site-specific integration and high-level expression of recombinant proteins in human cells. With built-in Zeocin™ selection and FRT recombination compatibility, it streamlines the generation of stable cell lines—making it ideal for a wide range of bioproduction, assay development, and functional research applications.