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Thermo Scientific™ Pierce™ Gentle Ag/Ab Elution Buffer
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Specifications:
| Application | Immunohistochemistry, Protein Purification | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
This elution buffer enables mild, non-acidic recovery of antibodies or antigen-antibody complexes by using near-neutral pH (6.6) combined with high ionic strength. Traditional elution methods using low-pH buffers can sometimes denature delicate proteins or compromise biological activity. In contrast, the Pierce™ Gentle Ag/Ab Elution Buffer preserves protein functionality and structure, making it suitable for sensitive downstream applications such as functional assays, enzyme activity measurements, or further binding interactions.
The buffer is phosphate-free, which avoids compatibility issues with other reagents (notably the Protein A Binding Buffer) that may precipitate under certain conditions.
Key Features & Benefits
-
Non-denaturing Elution
Maintains structural integrity of antibodies and antigens due to near-neutral pH. -
High-Salt Formulation
Uses ionic strength (instead of acid) to disrupt binding interactions gently. -
Pre-formulated & Ready to Use
Supplied as a filtered 1X liquid solution—no mixing or dilution required. -
Optimized for Gentle Ag/Ab Systems
Recommended for use with Pierce™ Gentle Ag/Ab Binding Buffer and ideal with Protein A/G affinity supports. -
Safe for Labile Proteins
Compatible with enzymes, functional antibodies, and other delicate biomolecules.
Specifications
| Feature | Details |
|---|---|
| Product Name | Pierce™ Gentle Ag/Ab Elution Buffer |
| pH | 6.6 |
| Buffer Type | High-salt (ionic strength) |
| Form | Liquid |
| Volume | 500 mL (Catalog No. 21027) |
| Storage Conditions | 4°C |
| Preservative | Contains sodium azide (0.05%) |
| Compatibility | Protein A, Protein G, Protein A/G columns |
| For Use With | Antibody or Ag/Ab affinity purification |
| Application | Non-denaturing protein elution |
Applications
- Elution of antibodies or antigen-antibody complexes from Protein A/G resins
- Antibody purification workflows requiring maintenance of protein function
- Gentle recovery of enzymatic or labile proteins from affinity matrices
- Compatible with downstream activity assays, binding studies, or structural characterization
Thermo Scientific™ Pierce™ Gentle Ag/Ab Elution Buffer (pH 6.6) provides an optimal alternative to harsh acidic elution protocols, enabling high-yield, non-denaturing purification of biologically active antibodies and antigen-antibody complexes. When used in tandem with the matching Gentle Ag/Ab Binding Buffer, this system supports sensitive applications that demand protein integrity, making it a critical tool in immunochemistry, therapeutic antibody development, and structural biology research.
- Pack Size: 500 mL 3.75L