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Thermo Scientific™ Pierce™ Protein A IgG Purification Kit
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Sufficient For: Purifying 6 to 8 mg mouse IgG per column
• Protein A Columns, 1 mL, 5 columns
• Protein A IgG Binding Buffer, 1 L
• IgG Elution Buffer, 500 mL
• Excellulose Desalting Columns, 5 mL, 5 columns
Specifications:
| Application | Protein Purification | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Pierce™ Protein A IgG Purification Kit is a ready-to-use, column-based system for the efficient purification of immunoglobulin G (IgG) antibodies. It utilizes high-quality Protein A Agarose resin, optimized buffers, and Excellulose desalting columns to ensure high yield and purity from serum, ascites fluid, or cell culture supernatants.
Protein A has high affinity for the Fc region of many mammalian IgG subclasses, allowing for selective antibody purification with minimal contamination. The agarose resin features immobilized native Protein A, covalently coupled via AminoLink™ chemistry for improved stability and low nonspecific binding.
Key Features & Benefits
- Complete Kit: Includes columns pre-packed with Protein A agarose, binding and elution buffers, and desalting columns—everything needed for fast, efficient antibody purification.
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High Binding Capacity:
- 12–19 mg of human IgG per mL of resin
- 6–8 mg of mouse IgG per mL of resin
- Species Compatibility: Effective for human, rabbit, pig, dog, and cat IgG. Note: weaker binding to mouse IgG1 and human IgG3.
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Optimized Buffers:
- Protein A IgG Binding Buffer (1 L)
- IgG Elution Buffer (500 mL)
- Low Non-specific Binding: The AminoLink chemistry ensures covalent immobilization of Protein A with minimal leaching and background signal.
- Reusable Resin: Durable for multiple purifications without loss of performance.
- Pre-packed Spin Columns: Convenient for quick processing and high reproducibility.
Kit Components
| Component | Quantity |
|---|---|
| Protein A Columns (1 mL) | 5 columns |
| Protein A IgG Binding Buffer | 1 L |
| IgG Elution Buffer | 500 mL |
| Excellulose Desalting Columns (5 mL) | 5 columns |
Resin & Column Specifications
| Property | Specification |
|---|---|
| Bead Type | Crosslinked 6% agarose (CL-6B) |
| Binding Capacity | 12–19 mg human IgG/mL; 6–8 mg mouse IgG/mL |
| Average Particle Size | 45–165 µm |
| pH Stability | 2–14 (short term); 3–13 (long term) |
| Max Flow Rate | ~1 mL/min (1 cm column diameter) |
| Max Pressure | <25 psi (1.5 bar) |
Applications
- Purification of polyclonal and monoclonal IgG antibodies
- Pre-clearing samples prior to immunoprecipitation
- Desalting and buffer exchange post-IgG purification
- Antibody cleanup for downstream assays (e.g., ELISA, western blotting, IHC)
The Pierce Protein A IgG Purification Kit is a comprehensive, high-performance tool for purifying IgG antibodies with convenience, consistency, and high yield. Its combination of optimized components and superior resin quality makes it suitable for both small-scale and preparative-scale applications in research laboratories. Whether you're working with serum, ascites, or culture supernatant, this kit simplifies the workflow and ensures reliable results with minimal background.