Thermo Scientific™ Pierce™ C18 Spin Columns

Pierce™ C18 Spin Columns are high-performance, ready-to-use peptide desalting and cleanup columns designed for mass spectrometry (MS) sample preparation. Packed with porous C18 reverse-phase resin, these microcentrifuge-compatible spin columns remove MS-incompatible salts, detergents, and other low molecular weight contaminants while concentrating peptide analytes for downstream MALDI-MS, ESI-MS, and LC-MS/MS workflows.

The optimized protocol enables efficient recovery of peptides derived from as little as 10 ng to 30 µg of digested protein, making these columns ideal for low-abundance proteomics, post-translational modification analysis, and peptide mapping applications.

Key Features & Benefits

• Efficient Desalting and Cleanup
  Removes common MS inhibitors such as SDS, urea, salts, and buffer components, improving signal-to-noise ratios and reproducibility.
• High Peptide Recovery
  Specialized C18 resin enables excellent retention and elution, even at sub-picomole peptide loads.
• Wide Sample Compatibility
  Accommodates a variety of sample volumes and concentrations without the need for volume reduction.
• Fast and Simple Workflow
  Complete sample processing in minutes using a standard centrifuge—no vacuum manifold or specialized equipment required.
• MS-Compatible Output
  Peptides are eluted in solvents directly compatible with MALDI, ESI, or LC-MS/MS analysis.

Applications

• Cleanup of protease-digested peptides before mass spectrometry
• Concentration and desalting of peptide samples from protein digests
• Sample preparation for MALDI-TOF, LC-MS/MS, or Orbitrap analysis
• High-throughput proteomics workflows requiring consistent and reproducible cleanup
• Analysis of post-translational modifications (PTMs) and peptide mapping

Technical Specifications

The Pierce™ C18 Spin Columns offer a reliable and efficient solution for peptide sample cleanup before MS. With excellent recovery, compatibility with common detection platforms, and rapid processing via centrifugation, they are ideal for low-input proteomics, biomarker discovery, and routine MS workflows.