Thermo Scientific™ Pierce™ C18 Spin Columns
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Specifications:
Application | Size Exclusion Chromatography, Mass Spectrometry |
Storage Temperature | 2-8°C |
Product Type | columns |
Product Brand | Thermo Fisher Scientific™ |
Product Grade | Molecular Biology |
Pierce™ C18 Spin Columns are high-performance, ready-to-use peptide desalting and cleanup columns designed for mass spectrometry (MS) sample preparation. Packed with porous C18 reverse-phase resin, these microcentrifuge-compatible spin columns remove MS-incompatible salts, detergents, and other low molecular weight contaminants while concentrating peptide analytes for downstream MALDI-MS, ESI-MS, and LC-MS/MS workflows.
The optimized protocol enables efficient recovery of peptides derived from as little as 10 ng to 30 µg of digested protein, making these columns ideal for low-abundance proteomics, post-translational modification analysis, and peptide mapping applications.
Key Features & Benefits
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Efficient Desalting and Cleanup
Removes common MS inhibitors such as SDS, urea, salts, and buffer components, improving signal-to-noise ratios and reproducibility. -
High Peptide Recovery
Specialized C18 resin enables excellent retention and elution, even at sub-picomole peptide loads. -
Wide Sample Compatibility
Accommodates a variety of sample volumes and concentrations without the need for volume reduction. -
Fast and Simple Workflow
Complete sample processing in minutes using a standard centrifuge—no vacuum manifold or specialized equipment required. -
MS-Compatible Output
Peptides are eluted in solvents directly compatible with MALDI, ESI, or LC-MS/MS analysis.
Applications
- Cleanup of protease-digested peptides before mass spectrometry
- Concentration and desalting of peptide samples from protein digests
- Sample preparation for MALDI-TOF, LC-MS/MS, or Orbitrap analysis
- High-throughput proteomics workflows requiring consistent and reproducible cleanup
- Analysis of post-translational modifications (PTMs) and peptide mapping
Technical Specifications
Attribute | Details |
---|---|
Product Type | Spin Column |
Resin Type | C18 reverse-phase porous resin |
Sample Compatibility | Peptides from protein digests |
Binding Capacity | 10 ng – 30 µg of peptides |
Format | Microcentrifuge spin column |
Workflow Step | Sample cleanup prior to MS |
Detection Method | Mass Spectrometry (MALDI, ESI, LC-MS/MS) |
Unit Sizes | 25 Columns (Cat. 89870), 50 Columns (Cat. 89873) |
For Use With | Microcentrifuge, Mass Spectrometer |
Storage Conditions | Room temperature |
Product Line | Pierce™ |
The Pierce™ C18 Spin Columns offer a reliable and efficient solution for peptide sample cleanup before MS. With excellent recovery, compatibility with common detection platforms, and rapid processing via centrifugation, they are ideal for low-input proteomics, biomarker discovery, and routine MS workflows.
- Pack Size: pack of 50 25/pk