Macherey-Nagel™ NucleoSpin™ Soil, Mini kit for DNA from soil

High-Yield, PCR-Ready DNA Extraction from Various Soil Types

The Macherey-Nagel™ NucleoSpin™ Soil Mini Kit is a high-efficiency DNA extraction kit designed for isolation of total DNA from soil, sediment, and sludge samples. Using silica membrane technology, this kit ensures maximum DNA yield while removing PCR inhibitors, making it ideal for environmental microbiology, metagenomics, and agricultural research.

With optimized lysis buffers, mechanical disruption via MN Bead Tubes Type A, and inhibitor removal columns, this kit efficiently extracts high-quality DNA from even the most challenging soil samples.

Key Features & Benefits

✅ High-Yield DNA Extraction (2–10 µg per 500 mg soil) – Recovers high-purity DNA suitable for molecular analysis

✅ Effective PCR Inhibitor Removal – Eliminates humic substances, polysaccharides, and other contaminants

✅ Fast & Simple Spin Column-Based Protocol – Uses centrifugation for efficient lysate clarification & DNA binding

✅ Two Lysis Buffer Options + Enhancer SX – Allows customized extraction conditions for different soil types

✅ Efficient Lysis for Tough-to-Disrupt Microorganisms – Suitable for bacterial, fungal, and archaeal DNA extraction

✅ Wide Sample Compatibility – Works with sediment, sludge, agricultural soil, and environmental samples

✅ Validated for PCR, qPCR, Microarrays & Southern Blotting – Ensures reliable downstream applications

Technical Specifications

Kit Components & Reagents

✔ Pre-Coated 96-Well Microplate (Capture Antibody)

✔ Standard (Recombinant HBsAg Antigen, Lyophilized)

✔ Biotinylated Detection Antibody

✔ HRP Conjugate

✔ Standard & Sample Diluent

✔ Biotinylated Detection Antibody Diluent

✔ HRP Conjugate Diluent

✔ Wash Buffer

✔ TMB Substrate

✔ Stop Solution

✔ Plate Sealer

Available Pack Sizes

How It Works

1️⃣ Lysis & Homogenization – Add lysis buffer & MN Bead Tubes Type A, followed by mechanical disruption.

2️⃣ Lysate Clarification – Use centrifugation & NucleoSpin Inhibitor Removal Column to remove inhibitors.

3️⃣ DNA Binding – Transfer clarified lysate to a NucleoSpin DNA column and bind DNA to the silica membrane.

4️⃣ Washing & Elution – Wash bound DNA to remove contaminants, then elute into 30–100 µL of buffer.

5️⃣ Downstream Analysis – DNA is now ready for PCR, qPCR, microarrays, and Southern blotting.

Applications

🔬 Environmental Microbiology & Metagenomics – Extracts DNA from complex soil microbial communities

🦠 Microbial Ecology Studies – Analyzes soil bacterial, fungal, and archaeal diversity

🌾 Agricultural & Soil Science Research – Investigates soil microbiomes & plant-microbe interactions

⚗️ Bioremediation & Pollution Studies – Monitors microbial communities in contaminated soils

🧪 PCR, qPCR, Microarrays & Southern Blotting – Ensures reliable molecular analysis of environmental DNA

Performance Validation

✅ PCR-Ready DNA from Various Soil Types – Ensures amplification success, even from undiluted eluates

✅ Enhanced Lysis for Tough Microorganisms – Extracts DNA from bacterial & fungal cell walls efficiently

✅ Consistently High Yields Across Sample Types – Works with clay-rich, organic, and sediment-heavy soils

Regulatory & Safety Information

⚠️ For Research Use Only. Not for diagnostic or clinical applications.

⚠️ Follow Safety Guidelines – Use proper PPE when handling samples & reagents.

⚠️ Store at 15–25°C to maintain optimal reagent stability.

Why Choose the NucleoSpin™ Soil Mini Kit?

🔹 High-Yield, High-Purity DNA Extraction from Soil

🔹 Validated for Environmental & Microbiome Studies

🔹 Removes PCR Inhibitors for Reliable Amplification

🔹 Two Lysis Buffers for Optimal Extraction Conditions

🔹 Fast & Efficient Processing (90 Min for 10 Preps)

For bulk orders, technical support, or additional information, contact LabMart Limited today.