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Applied Biosystems™ MagMAX™ Microbiome Ultra Nucleic Acid Isolation Kit, with bead tubes, for 100 preps
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Specifications:
| Application | Genomic Purification |
| Storage Temperature | Room Temperature |
| Product Type | Sample Prep |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
The MagMAX™ Microbiome Ultra Nucleic Acid Isolation Kit (with bead tubes) is a specialized magnetic bead–based purification system designed for efficient and reproducible RNA and DNA extraction from challenging microbiome-rich samples, particularly stool, soil, and swabs. Leveraging the proven performance of MagMAX magnetic bead technology and optimized for use on KingFisher™ Flex and KingFisher™ Duo Prime instruments, this kit delivers high-quality nucleic acids suitable for downstream RT-PCR, qPCR, microarrays, and next-generation sequencing (NGS) applications.
This version of the kit includes pre-filled bead tubes, making it ideal for low- to medium-throughput workflows that require flexibility without sacrificing yield or quality. With optimized surface chemistry via Dynabeads™ MyOne™ Silane, the kit offers excellent binding efficiency and avoids issues common with filter-based methods such as clogging.
Key Features
Optimized for Difficult Sample Types
- Validated for extraction from fecal matter, soil, and swabs in transport media.
- Processes up to 100 mg of stool or 250 mg of soil per prep.
- Ideal for microbiome and environmental nucleic acid studies.
Bead Tube Format for Low-Throughput Labs
- Includes individual tubes pre-loaded with zirconia beads for efficient mechanical disruption.
- Suitable for smaller labs or batch processing needs.
- After bead beating, samples can be processed on a KingFisher™ instrument.
High-Quality Magnetic Bead Chemistry
- Dynabeads™ MyOne™ Silane feature a silica-like surface with high surface area for superior nucleic acid binding.
- Eliminates common issues such as filter clogging seen with column- or vacuum-based kits.
- Supports elution volumes from 50 to 200 μL, with consistent RNA/DNA recovery in as little as 100 μL of elution buffer.
Rapid and Efficient Workflow
- Processes up to 96 samples in ~60 minutes on KingFisher™ Flex, or 12 samples in ~50 minutes on KingFisher™ Duo Prime.
- Pre-optimized automation-ready protocols simplify validation and ensure consistent performance.
Specifications
| Attribute | Details |
|---|---|
| Isolation Technology | Magnetic Bead (Dynabeads™ MyOne™ Silane) |
| Final Product Type | RNA and DNA |
| Sample Types | Bacteria, Feces, Soil, Swabs (Buccal or Skin), Yeast, Biofluids |
| Elution Volume | 50 to 200 μL |
| Starting Material | Feces ≤100 mg, Soil ≤250 mg, Biofluids ≤500 μL, Yeast ≤500 μL |
| Test Time | ~60 min (KingFisher™ Flex), ~50 min (KingFisher™ Duo Prime) |
| Automation Compatibility | KingFisher™ Flex, Duo Prime (after bead beating step) |
| For Use With Applications | RT-PCR, qPCR, NGS, microarray, blotting, hybridization assays |
| High-throughput Compatibility | Manual bead beating format; post-disruption suitable for automation |
| Storage Condition | Room Temperature |
| Kit Contents | Lysis Buffer, Binding Solution, Wash Buffer, Elution Solution, Proteinase K, Binding Beads, Bead Tubes (100x) |
Comparison with Other MagMAX™ Nucleic Acid Kits
| Kit Name | Throughput Format | Sample Types | Key Features |
|---|---|---|---|
| MagMAX™ Microbiome Ultra Kit (Bead Tubes) | Low-throughput (Manual) | Stool, soil, swabs | Individual tubes with zirconia beads; manual bead beating; automation-ready |
| MagMAX™ Microbiome Ultra Kit (Plate Format) | High-throughput (96-well) | Stool, soil, swabs | Pre-filled 96-well plate with zirconia beads; optimized for KingFisher Flex |
| MagMAX™ CORE Nucleic Acid Purification Kit | Universal throughput | 15+ veterinary/environmental types | Modular design, room temp storage, universal protocol across KingFisher systems |
Unique Advantages of the MagMAX™ Microbiome Ultra Kit (Bead Tubes)
- Designed for Challenging Microbiome Samples: Processes unfiltered and particulate-rich samples like feces and soil with high efficiency.
- Superior Magnetic Bead Chemistry: Dynabeads™ MyOne™ Silane provide high nucleic acid recovery with excellent purity.
- Manual Flexibility + Automation Compatibility: Ideal for labs that need flexibility in sample prep, with automation-ready lysate post-disruption.
- Reduced Workflow Downtime: Eliminates filter clogging and reduces prep time for cleaner, faster results.
- Supports Broad Application Range: RNA and DNA are suitable for qPCR, RT-PCR, sequencing, microarrays, and more.