Liofilchem® Nutrient Agar ISO 16266

The Liofilchem® Nutrient Agar ISO 16266 is a high-quality microbiological medium formulated for cultivating non-fastidious organisms and confirming the presence of Pseudomonas aeruginosa in water samples. It adheres to the guidelines specified in ISO 16266 for detecting and enumerating P. aeruginosa using the membrane filtration technique. Available in multiple formats, including plates, bottles, slant tubes, and dehydrated media, it is suitable for professional laboratory environments.

Key Features

1. ISO 16266 Compliance:
   • Designed specifically for water microbiology testing to detect and enumerate Pseudomonas aeruginosa.
2. Broad Applicability:
   • Ideal for cultivating non-fastidious organisms from a variety of water samples.
3. Versatile Formats:
   • Available in ready-to-use plates, slant tubes, and dehydrated media, providing flexibility for different laboratory workflows.
4. High-Quality Composition:
   • Carefully formulated to ensure consistent and reliable growth of target organisms.
5. Long Shelf Life:
   • Dehydrated medium: 4 years.
   • Ready-to-use plates: 6 months.

Typical Formula

Applications

1. Water Microbiology:
   • Detection and enumeration of Pseudomonas aeruginosa in water using the membrane filtration method.
2. Environmental Monitoring:
   • Ideal for testing non-fastidious bacteria in water from environmental and industrial sources.
3. General Microbiology:
   • Suitable for cultivating a wide range of non-fastidious bacterial species.

Preparation Instructions

1. Dehydrated Medium:
   • Suspend 28 g of powder in 1 liter of distilled or deionized water.
   • Heat to boiling, shaking frequently until completely dissolved.
   • Sterilize in an autoclave at 121°C for 15 minutes.
2. Ready-to-Use Bottles:
   • Melt the medium in a water bath at 100°C with the cap partially loosened.
   • Mix well, avoiding foam formation, and distribute into sterile Petri dishes or other containers.

Test Procedure

1. Transfer the membrane with presumptive Pseudomonas aeruginosa onto the medium.
2. Incubate at 36 ± 2°C for 20-24 hours aerobically.
3. Observe colony growth and confirm P. aeruginosa presence with the oxidase test.

Interpretation of Results

• Growth Observation:
  • Good growth of Pseudomonas aeruginosa is indicated by distinct colonies.
  • Confirmation is performed via oxidase test (ref. 88029).
• Control Strains:
  • Pseudomonas aeruginosa: WDCM 00025 (ATCC 27853).
  • Escherichia coli: WDCM 00013 (ATCC 25922).

Storage and Stability

• Dehydrated Medium: Store at 10-30°C in a dry, sealed container.
• Prepared Media: Store plates, tubes, and bottles at 10-25°C away from light.
• Avoid temperature fluctuations to prevent condensation on plates.

Quality Control

Available Formats

Advantages

1. ISO 16266 Compliant:
   • Ensures adherence to international water testing standards.
2. Versatility:
   • Supports membrane filtration, spread plating, and direct streaking.
3. Quality Assured:
   • QC-tested with control strains for reliability and consistency.
4. Extended Shelf Life:
   • Dehydrated medium and ready-to-use formats maintain long-term usability.
5. Wide Availability:
   • Offered in multiple formats and pack sizes to meet diverse laboratory needs.

The Liofilchem® Nutrient Agar ISO 16266 is a reliable and standardized medium for cultivating non-fastidious organisms and confirming the presence of Pseudomonas aeruginosa in water samples. With multiple formats, long shelf life, and adherence to international standards, it is an essential tool for water microbiology testing and environmental monitoring.