Liofilchem® Listeria System 18 R

The Liofilchem® LISTERIA SYSTEM 18R is a robust and compact 18-well microbiological testing system designed for the biochemical identification of Listeria spp.. This system is a rapid and reliable tool for microbiology laboratories, enabling the presumptive identification of Listeria monocytogenes and related species from cultures grown on selective or non-selective agar media. Results are provided within 18-24 hours, with additional confirmatory tests to ensure precision and accuracy.

Key Features:

1. Multi-Well Biochemical Identification:
   • Detects and identifies various Listeria species through 18 biochemical tests:
     • Listeria monocytogenes
     • Listeria innocua
     • Listeria grayi subsp. grayi
     • Listeria grayi subsp. murrayi
     • Listeria seeligeri
     • Listeria welshimeri
     • Listeria ivanovii
     • Jonesia denitrificans
2. Fast Results:
   • Results are delivered in as little as 18-24 hours, ensuring rapid identification.
3. Numerical Code Identification:
   • Interpretation of results generates a 6-digit numerical code for precise identification, using predefined biochemical profiles.
4. Validated and Reliable:
   • Each system is validated for accuracy and subjected to rigorous quality control using reference strains.
5. Ease of Use:
   • Pre-configured wells with dried biochemical substrates simplify preparation and interpretation.
6. Supplementary Confirmatory Tests:
   • Includes additional tests, such as ABN, indole, catalase, methyl red, nitrate reduction, and VP tests for complete microbial identification.

Specifications:

Kit Components:

• 20 LISTERIA SYSTEM 18R panels
• 20 Vials of Physiological Solution
• 20 Xylose Discs
• 20 ABN Discs
• 1 Vial of ABN Reagent (3 mL)
• Instruction Sheet

Additional Items Required (Not Included):

• LISTERIA SYSTEM 18R Reagent (Code 80257)
• Gram Color Kit (Code 80293)
• Vaseline Oil (Code 80278)
• Sundry laboratory materials (slides, cover slips, microscope).

Applications:

1. Food Safety Testing:
   • Identify Listeria spp. in food samples such as meat, dairy, and processed foods.
2. Microbial Screening:
   • Perform rapid and accurate biochemical identification of Listeria species from selective/non-selective agar cultures.
3. Clinical and Environmental Microbiology:
   • Screen samples from clinical or environmental sources for Listeria contamination.

How It Works:

1. Preparation of Bacterial Suspension:
   • Observe colony morphology and confirm the sample belongs to the Listeria genus with Gram staining.
   • Prepare a bacterial suspension using Physiological Solution provided in the kit.
2. System Inoculation:
   • Inoculate each well with 0.2 mL (4 drops) of the suspension.
   • Add Xylose Disc to well 15 and cover wells 2 (UR) and 3 (H2S) with Vaseline Oil.
   • Incubate at 36°C ± 1°C for 18-24 hours.
3. Additional Tests:
   • Conduct confirmatory tests, such as:
     • ABN Test: Identifies non-monocytogenes species by observing yellow coloration in well 9-ABN.
     • Indole Test: Add Kovac's reagent to well 4-IND.
     • Catalase Test: Add H2O2 to well 6-CAT.
     • Methyl Red Test: Add Methyl Red reagent to well 7-MR.
     • Nitrate Test: Add Sulfanilic Acid and Naftilamine to well 17-NIT.
     • VP Test: Add Alpha-Naphthol and NaOH 40% to well 18-VP.
4. Result Interpretation:
   • Observe color changes in the wells and interpret using the included Numerical Code Table.
   • Use the 6-digit numerical code to identify the organism from the Identification Table.

Key Benefits:

1. Compact and Comprehensive:
   • Performs 18 biochemical tests in a single panel, saving time and resources.
2. Accurate and Reliable:
   • Validated system with a high degree of accuracy for Listeria spp. identification.
3. Flexible Testing:
   • Supports additional biochemical and confirmatory testing for complete microbial profiling.
4. Fast Results:
   • Provides results in less than 24 hours, expediting decision-making in food safety and clinical diagnostics.

Validation and Quality Control:

• Validated against reference strains, including:
  • Listeria monocytogenes (ATCC 35152)
  • Listeria innocua (ATCC 33090)
  • Listeria ivanovii (ATCC 19119)
• Quality control ensures reliable performance across batches.

Precautions:

• Ensure all materials are sterile and procedures are followed as per instructions.
• Use by trained personnel in a laboratory setting.
• Store at 2°C to 8°C and do not use expired systems.

Storage and Disposal:

• Store the kit at 2°C to 8°C in its original packaging.
• Dispose of used systems and associated materials following standard laboratory decontamination protocols.

References:

1. McLannchlin, Identification of Listeria species, International Journal of Food Microbiology, 38 (1997) 77–81.
2. Bailey and Scott’s Diagnostic Microbiology, 7th ed., C.V. Mosby Company, 1986.
3. Edwin H. Lenette, Manual of Clinical Microbiology, 4th ed., ASM Washington, 1985.

For Research Use Only:

This product is intended for research purposes only and is not approved for diagnostic or therapeutic use.