Liofilchem® Listeria System 18R Reagents, 100-200 Tests

The Liofilchem® Listeria System 18R Reagent is a comprehensive reagent kit specifically designed for use with the LISTERIA SYSTEM 18R. This reagent kit contains all necessary solutions for performing biochemical identification tests on Listeria spp., including tests for Voges-Proskauer, catalase, indole, nitrate reduction, methyl red, and more. It ensures accurate, reliable, and efficient biochemical identification of Listeria monocytogenes and related species.

Key Features:

1. Comprehensive Kit:
   • Includes reagents for all biochemical tests required in the Listeria System 18R:
     • Alpha Naphtol
     • NaOH 40%
     • Hydrogen Peroxide
     • Methyl Red
     • Sulphanilic Acid
     • Alpha Naphthylamine
     • Kovac's Reagent
     • Vaseline Oil
2. Supports Multiple Tests:
   • Facilitates the execution of key tests, such as:
     • Voges-Proskauer (VP) (Well 18-VP)
     • Catalase Test (Well 6-CAT)
     • Methyl Red Test (Well 7-MR)
     • Nitrate Test (Well 17-NIT)
     • Indole Test (Well 4-IND)
     • Anaerobic maintenance with Vaseline Oil (Wells 2-UR and 3-H2S).
3. Easy Integration:
   • Tailored for seamless use with the Listeria System 18R, simplifying workflows for accurate identification of Listeria spp..
4. High Capacity:
   • Sufficient for 100-200 tests, depending on usage.
5. Validated and Reliable:
   • Each reagent batch undergoes rigorous quality control, ensuring consistency and reliability.

Kit Contents:

Applications:

1. Listeria Identification:
   • Facilitates accurate biochemical identification of Listeria spp., including:
     • Listeria monocytogenes
     • Listeria innocua
     • Listeria ivanovii, and more.
2. Biochemical Testing:
   • Supports multiple biochemical tests for confirmatory identification:
     • Voges-Proskauer Test for acetoin production.
     • Catalase Test for enzyme detection.
     • Indole Test for tryptophan metabolism.
     • Nitrate Reduction Test for anaerobic respiration.
     • Methyl Red Test for acid production.
3. Food Safety and Microbial Research:
   • Ideal for microbiology labs involved in food safety testing, clinical microbiology, and environmental microbiology research.

How It Works:

1. Sample Preparation:
   • Prepare bacterial suspension using the Listeria System 18R protocol.
2. Biochemical Testing:
   • Add the appropriate reagents to specific wells of the Listeria System 18R as per instructions:
     • Add Alpha Naphtol and NaOH 40% to Well 18-VP for the Voges-Proskauer test.
     • Add Hydrogen Peroxide to Well 6-CAT for the Catalase test.
     • Add Kovac's Reagent to Well 4-IND for the Indole test.
     • Add Sulphanilic Acid and Alpha Naphthylamine to Well 17-NIT for the Nitrate Reduction test.
     • Add Methyl Red to Well 7-MR for the Methyl Red test.
3. Result Interpretation:
   • Observe color changes or gas formation in the wells to interpret results:
     • Positive reactions for biochemical tests are indicated by specified color changes or bubbles.

Reagent Composition:

Key Benefits:

1. Comprehensive Solution:
   • All necessary reagents for Listeria biochemical testing in a single kit.
2. High Capacity:
   • Supports 100-200 tests, making it suitable for high-throughput laboratories.
3. Accuracy and Consistency:
   • Each reagent undergoes stringent quality control to ensure reliable and reproducible results.
4. Easy Integration:
   • Tailored for use with the Listeria System 18R, simplifying workflows and reducing errors.
5. Time-Saving:
   • Streamlines confirmatory testing for Listeria spp., reducing turnaround time.

Storage and Handling:

• Store at 2-8°C away from light.
• Discard if signs of deterioration or contamination are evident.

Precautions:

• For professional use only.
• Ensure proper aseptic techniques and safety measures are followed when handling reagents.
• Refer to the Safety Data Sheet (SDS) for detailed handling and hazard information.

Quality Control:

• Perform positive and negative quality control checks for each reagent using suitable microorganisms.
• Refer to the literature for expected results with control strains.

Kit Presentation:

References:

1. Murray, Baron, Pfaller, Tenorev, Yolken: Manual of Clinical Microbiology, 1995.
2. Bayley and Scott’s: Diagnostic Microbiology, 1986.
3. Edwin H. Lenette: Manual of Clinical Microbiology, 1995.

For Research Use Only:

This product is intended for research purposes only and is not approved for diagnostic or therapeutic use.