Liofilchem® Integral System Stafilococchi

The Liofilchem® INTEGRAL SYSTEM STAFILOCOCCHI is a 24-well colorimetric system designed for the biochemical identification and antimicrobial susceptibility testing (AST) of staphylococci. This innovative tool provides reliable, efficient, and simultaneous identification and susceptibility results within 18-24 hours, making it an essential addition to microbiological laboratories.

Key Features:

1. Dual Functionality:
   • Performs biochemical identification (Wells 1–8) and antimicrobial susceptibility testing (AST) (Wells 9–24) simultaneously.
2. Comprehensive Biochemical Tests:
   • Identifies Staphylococcus species through specific substrate-based reactions:
     • Arginine decarboxylation (ADC)
     • Urea hydrolysis (UR)
     • Mannitol, sucrose, maltose, and trehalose fermentation, among others.
3. Antibiotic Susceptibility Testing:
   • Tests against a wide range of antibiotics, including:
     • Gentamicin, Tobramycin, Amoxicillin-Clavulanic Acid, Ampicillin-Sulbactam, Ciprofloxacin, and others.
4. Colorimetric Results:
   • Results are easy to interpret via color changes in the wells:
     • Yellow: Growth (Resistant).
     • Red: Inhibited growth (Sensitive).
5. Numerical Coding System:
   • Generates a three-digit code from the biochemical test results, which is matched against a database for precise species identification.
6. Validated and Reliable:
   • Complies with NCCLS guidelines and is validated using reference strains (e.g., ATCC).

Specifications:

Kit Components:

• 20 INTEGRAL SYSTEM STAFILOCOCCHI panels
• 20 Xylose Discs
• 20 Vials of Inoculum Broth (7.0 mL/vial)
• Instruction Manual

Additional Items Required (Not Included):

• Vaseline Oil (Code 80278)
• Physiological Solution for Microbiological Use (Code 20095)
• Standard laboratory supplies (pipettes, incubator, etc.)

Applications:

1. Clinical Microbiology:
   • Identify and determine antibiotic susceptibility of Staphylococcus species isolated from clinical samples.
2. Food Microbiology:
   • Detect and test staphylococcal contamination in food and environmental samples.
3. Pharmaceutical Research:
   • Evaluate the efficacy of antimicrobial compounds against staphylococcal strains.
4. Quality Control:
   • Perform routine microbial testing in laboratories adhering to regulatory standards.

How It Works:

1. Sample Preparation:
   • Isolate Staphylococcus colonies from selective/non-selective agar and prepare a bacterial suspension equivalent to 0.5 McFarland in physiological solution.
2. System Inoculation:
   • Biochemical Identification:
     • Add 0.2 mL of bacterial suspension into wells 1-8.
     • Add Vaseline Oil to wells 1 (ADC) and 2 (UR).
     • Insert a Xylose Disc into well 6 (XYL).
   • Antimicrobial Susceptibility Testing:
     • Add 0.2 mL of bacterial suspension into the provided Inoculum Broth, then distribute 0.2 mL into wells 9–24.
3. Incubation:
   • Cover the system and incubate at 36°C ± 1°C for 18-24 hours.
4. Result Interpretation:
   • Biochemical Identification:
     • Evaluate color changes in wells 1-8 and generate a three-digit code to identify the organism.
   • Antibiotic Susceptibility:
     • Observe growth inhibition in wells 9–24. Interpret as:
       • Sensitive (S): Red.
       • Intermediate (I): Orange.
       • Resistant (R): Yellow.

Key Benefits:

1. Time-Saving:
   • Simultaneously performs identification and susceptibility testing in one system, delivering results within a single incubation cycle.
2. Wide Antibiotic Panel:
   • Includes 15 antibiotics with NCCLS-compliant concentrations, allowing for comprehensive susceptibility profiling.
3. Accurate Identification:
   • Generates a three-digit code for precise bacterial species identification.
4. Ease of Use:
   • Pre-configured wells and simple protocols streamline workflow for microbiology labs.
5. Reliable and Validated:
   • Validated against traditional methods, ensuring high accuracy and reproducibility.

Antibiotics Included (Wells 9–24):

• Gentamicin (CN)
• Tobramycin (TOB)
• Amoxicillin-Clavulanic Acid (AUG)
• Ampicillin-Sulbactam (AMS)
• Ceftazidime (CAZ)
• Cefuroxime (CXM)
• Ciprofloxacin (CIP)
• Levofloxacin (LEV)
• Azithromycin (AZM)
• Clarithromycin (CLR)
• Erythromycin (ERY)
• Roxithromycin (ROX)
• Fosfomycin (FOS)
• Rifampicin (RD)
• Co-Trimoxazole (SXT)

Validation and Quality Control:

• Tested against ATCC reference strains, including:
  • Staphylococcus aureus (ATCC 25923)
  • Staphylococcus epidermidis (ATCC 12228)
  • Staphylococcus xylosus (ATCC 35033).

Precautions:

• For in vitro diagnostic use only.
• Perform testing in a professional laboratory environment.
• Follow all biosafety guidelines for handling pathogenic microorganisms.

Storage and Handling:

• Store the kit at 2–8°C in its original packaging.
• Do not use expired kits or reagents.

References:

1. NCCLS, Performance Standards for Antimicrobial Susceptibility Testing, 2004.
2. Kloos W.E., Jorgensen J.H., Manual of Clinical Microbiology, 4th ed.
3. Bauer A.W. et al., Antimicrobic Susceptibility Testing by a Standardized Single Disk Method, Am. J. Clin. Pathol., 1966.

For Research Use Only:

This product is intended for research purposes only and is not for therapeutic or diagnostic use.