Liofilchem Physiological Solution 20 vials x 7 mL

The Physiological Solution by Liofilchem® is an isotonic diluent formulated to maintain cell integrity and viability in laboratory procedures requiring the preparation of microbial suspensions. This sterile solution contains 0.85% Sodium Chloride (NaCl), dissolved in water, and is designed to offer osmotic protection for microbial cells. Its versatility makes it a critical tool in a wide array of microbiological and diagnostic applications.

Key Features:

1. Isotonic Composition: Contains 0.85% NaCl to provide optimal osmotic conditions for maintaining cell integrity and viability.
2. Sterile and Ready-to-Use: Ensures reliability in laboratory settings while minimizing preparation steps.
3. Widely Applicable: Recommended for:
   • Preparing bacterial suspensions and adjusting turbidity to match McFarland inoculum standards.
   • Stock solution preparation and serial dilutions for antimicrobial susceptibility testing.
4. Compliance with Standards: The solution aligns with guidelines from:
   • CLSI (Clinical and Laboratory Standards Institute).
   • EUCAST (European Committee on Antimicrobial Susceptibility Testing).
5. Long Shelf Life: The solution has a stability period of 5 years, offering extended usability when stored appropriately.
6. Non-Hazardous Composition: Free from hazardous substances in concentrations above the legal thresholds.

Applications:

• Microbial Suspension Preparation: Used for inoculum standardization and maintaining cell viability during testing.
• Antimicrobial Susceptibility Testing: Essential for serial dilutions and preparing stock solutions of antimicrobial agents.
• Laboratory Diagnostics: Supports procedures in clinical microbiology, food testing, and pharmaceutical quality control.

Composition (per liter):

• Sodium Chloride (NaCl): 8.5 g
• pH: 7.0 ± 0.2 at 25°C

Preparation and Use:

1. Test Procedure: Inoculate the solution with 1–5 isolated colonies of the test organism.
2. Standardization: Adjust suspension turbidity to meet the desired McFarland standard.
3. Application: Use for culture preparation, serial dilutions, or as a diluent for bacterial suspensions.

Quality Control:

• Microbial Growth on Tryptic Soy Agar (TSA):
  • Escherichia coli (ATCC® 25922): Growth within ±30% of original count.
  • Staphylococcus aureus (ATCC® 29213): Growth within ±30% of original count.

Packaging Options:

• Tubes: 3 ml, 9 ml, or 10 ml volumes.
• Vials: 4.5 ml, 7 ml.
• Bottles: Ranges from 38 ml to 1,000 ml.

Storage Instructions:

• Store between 2°C and 30°C in its original packaging, protected from light.
• Discard if contamination or deterioration is observed.

Advantages:

1. Sterile and Reliable: Ensures accurate results across applications.
2. Versatile: Compatible with diverse microbiological workflows, including antimicrobial susceptibility testing.
3. Time-Saving: Ready-to-use solution minimizes preparation time.
4. Professional Standards Compliance: Meets global standards for laboratory testing.

Precautions:

• Intended for in vitro diagnostic use.
• For professional use only, handled by trained laboratory personnel.
• Dispose of according to local and national waste regulations.

Additional Information:

The Physiological Solution supports a wide range of microbiological testing and diagnostic processes, providing a robust and standardized medium for microbial handling. Its compliance with international standards makes it a trusted choice for clinical laboratories, research institutions, and quality control environments.