Liofilchem® FOOD SYSTEM

The Liofilchem® Food System (Ref. 71680) is an innovative 24-well microbiological system designed for the detection and presumptive identification of pathogenic microorganisms from food samples, including meat, dairy, fish, and other foodstuffs. It offers a compact, efficient, and cost-effective solution for microbiological screening, delivering reliable results within 18-24 hours. Validated to ISO 16140 standards, the Food System provides laboratories with a simple yet powerful method for food safety testing.

Key Features:

1. Simultaneous Multi-Microorganism Detection:
   • Detects and identifies several key foodborne pathogens, including:
     • Salmonella spp.
     • Proteus/Providencia spp.
     • Pseudomonas spp.
     • Staphylococcus aureus
     • Escherichia coli and E. coli O157
     • Bacillus cereus
     • Listeria spp.
     • Yeasts and molds
2. Validated for Accuracy:
   • ISO 16140-certified for the detection of Salmonella spp. and Listeria spp., ensuring compliance with international standards.
3. Rapid Results:
   • Provides results within 18-24 hours of incubation at 36°C ± 1°C.
4. Easy-to-Use:
   • Straightforward protocol involving inoculation, incubation, and interpretation through color changes in the wells.
5. Dual-Sample Testing:
   • Each system allows testing of two separate samples or duplicate testing of a single sample.
6. Cost-Effective:
   • Reduces labor, consumables, and the need for complex instruments while providing reliable and reproducible results.
7. Customizable Confirmatory Testing:
   • Enables additional confirmatory biochemical, immunological, or microscopic tests for accurate microorganism identification.

Specifications:

Applications:

1. Food Safety and Quality Control:
   • Detect foodborne pathogens in raw materials, production lines, and finished products.
2. Compliance with Food Standards:
   • Ensures adherence to food safety standards, including ISO and HACCP regulations.
3. Microbial Screening:
   • Screen for contamination in various food matrices with ease.
4. Foodborne Disease Research:
   • Analyze foodborne pathogen behavior and validate food safety procedures.

How It Works:

1. Sample Preparation:
   • Homogenize a food sample in Buffered Peptone Water (BPW) or Ringer's Solution to create a 1:10 dilution.
   • Incubate the sample suspension at 36 ± 1°C for 12-18 hours.
2. System Inoculation:
   • Add 0.5 mL of the sample suspension into the provided Physiological Solution.
   • Distribute 0.2 mL of the suspension into each well.
3. Incubation:
   • Cover specific wells with Vaseline Oil (e.g., LDC, H2S, UR) and incubate at 36 ± 1°C for 18-24 hours.
4. Result Interpretation:
   • Observe color changes in the wells to detect and presumptively identify microorganisms.
   • For example:
     • Salmonella spp.: Yellow to red/orange (LDC) or yellow to black (H2S).
     • E. coli: Red to blue (ESC) with pink-red after adding Kovac’s Reagent (IND).
     • Listeria spp.: Yellow to black (LIS) and bubble formation in CAT after adding H2O2.
5. Optional Confirmation:
   • Perform biochemical or serological tests (e.g., oxidase test for Pseudomonas spp.) or plate onto selective agar for further confirmation.

Included Components:

1. 20 Food System Panels (40 Tests)
2. 40 Vials of Physiological Solution (4.5 mL/vial)
3. 20 Sealing Films
4. Instruction Manual
5. 1 Test Result Form

Items Not Included (But Necessary for Use):

• Buffered Peptone Water (BPW)
• Vaseline Oil
• Oxidase Test Stick
• Kovac’s Reagent
• H2O2 Reagent
• Microscope and accessories

Validation:

The Liofilchem® Food System has undergone rigorous validation, with comparative studies showing high levels of accuracy, sensitivity, specificity, and selectivity when tested against ISO methods.

• Relative Accuracy: 94.0–97.2%
• Relative Sensitivity: 85.7–96.8%
• Relative Specificity: 97.6–100%
• Selectivity: 100%

Key Benefits:

1. Reliable and Validated:
   • Independently validated by the Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise.
2. Compact Design:
   • Test multiple microorganisms simultaneously in a single, compact panel.
3. Simplifies Workflow:
   • Eliminates the need for extensive equipment or training.
4. Supports Confirmatory Testing:
   • Easily extract samples for additional biochemical, immunological, or serological testing.

References:

1. Difco & BBL Manual, Manual of Microbiological Culture Media 2003.
2. ISO 6579:2002. Horizontal method for the detection of Salmonella spp.
3. ISO 6888-2:1999. Enumeration of coagulase-positive Staphylococci.
4. ISO 7932:2004. Horizontal method for the enumeration of presumptive Bacillus cereus.
5. ISO 11290:2002. Horizontal method for the detection and enumeration of Listeria monocytogenes.

For Research Use Only:

This product is intended for research purposes only and is not for diagnostic or therapeutic use.