0
Invitrogen™ NuPAGE™ Tris-Acetate SDS Buffer Kit
Catalog No :
CAS Number :
Brand :
In Stock
kit includes;
• NuPAGE Tris-Acetate SDS Running Buffer (20X, Cat. No. LA0041)
• NuPAGE Sample Reducing Agent (10X, 250 μL, Cat. No. NP0004)
• NuPAGE Antioxidant (Cat. No. NP0005)
• NuPAGE LDS Sample Buffer (4X, 10 mL, Cat. No. NP0007)
Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ NuPAGE™ Tris-Acetate SDS Buffer Kit is a comprehensive buffer system designed specifically for the efficient separation of medium to large molecular weight proteins on NuPAGE™ Tris-Acetate Gels. This ready-to-use kit includes four essential reagents needed to prepare and run your SDS-PAGE workflow with confidence, ensuring optimal resolution and protein integrity.
The included buffers and reducing agents support denaturing electrophoresis, minimizing sample degradation and disulfide interference. Together, they offer a complete and optimized electrophoresis solution for researchers working with high-molecular-weight proteins.
Kit Components
| Component | Catalog No. | Volume | Storage Temperature |
|---|---|---|---|
| 20X Tris-Acetate SDS Running Buffer | LA0041 | 500 mL | 4°C to 25°C |
| 10X Sample Reducing Agent (500 mM DTT) | NP0004 | 250 μL | 2°C to 8°C |
| NuPAGE™ Antioxidant | NP0005 | 15 mL | 4°C |
| 4X LDS Sample Buffer | NP0007 | 10 mL | 4°C to 25°C |
Key Features and Benefits
- Complete kit for NuPAGE™ Tris-Acetate SDS-PAGE workflows
- Supports separation of medium-to-large proteins (ideal for >200 kDa)
- Neutral pH system reduces protein degradation and increases resolution
- Includes reducing agent and antioxidant to maintain protein integrity during electrophoresis
- Ready-to-use formats – no additional mixing or pH adjustments needed
Applications
- SDS-PAGE analysis of medium to high molecular weight proteins
- Western blotting and downstream proteomic analysis
- Routine and high-throughput protein size separation
- Sample preparation for mass spectrometry
Compatible Products
- NuPAGE™ Tris-Acetate Precast Gels
- NuPAGE™ Novex Mini Cell & Mini Gel Tank
- iBlot™ 2 or Power Blotter for Western transfer
- NuPAGE™ Prestained Protein Standards
The NuPAGE™ Tris-Acetate SDS Buffer Kit delivers all the essential reagents needed for optimal separation and analysis of larger proteins using SDS-PAGE. With simplified workflow support and reagents formulated to enhance resolution, reduce oxidation, and protect protein structure, this kit is a critical component for researchers demanding precision in protein separation and blotting workflows.