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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Human MMP2 ELISA Kit is a high-performance solid-phase sandwich enzyme-linked immunosorbent assay designed for the quantitative detection of human Matrix Metalloproteinase-2 (MMP2) in biological samples. This assay provides sensitive and reproducible measurement of both natural and recombinant human MMP2 in serum, plasma, buffered solutions, and cell culture supernatants.
MMP2 is a key protease involved in extracellular matrix degradation, tissue remodeling, angiogenesis, and disease progression in cancer, inflammatory disorders, and connective tissue pathologies. This kit is rigorously validated for accuracy, precision, and lot-to-lot consistency, making it ideal for research and biomarker studies.
Key Features
- Quantitative detection of human MMP2 (Gelatinase A)
- Solid-phase sandwich ELISA format
- Recognizes both recombinant and native human MMP2
- High sensitivity with broad dynamic assay range
- Suitable for serum, plasma, and cell culture supernatants
- Validated for precision and reproducibility
- Compatible with standard absorbance microplate readers
Principle of Operation
This ELISA is based on a matched antibody pair sandwich method:
- A target-specific capture antibody is pre-coated onto the microplate wells
- Samples, standards, and controls are added and bind to the capture antibody
- A biotinylated detection antibody binds the captured MMP2
- Streptavidin-HRP conjugate is added
- Substrate reacts to generate a measurable color signal
- Signal intensity is proportional to the MMP2 concentration in the sample
Kit Performance Specifications
| Parameter | Specification |
|---|---|
| Analytical Sensitivity | < 1 ng/mL |
| Assay Range | 0.78 – 50 ng/mL |
| Assay Format | Sandwich ELISA |
| Time to Result | ~4 hours |
| Hands-on Time | ~1 hour 20 minutes |
| Interassay CV | 5.6% |
| Intraassay CV | 3.3% |
| Detection Method | Absorbance |
| Instrument Required | Microplate Reader |
Sample Compatibility
| Sample Type | Volume Required |
|---|---|
| Serum | 50 µL |
| Plasma | 50 µL |
| Cell Culture Supernatant | 50 µL |
Kit Contents
Each kit includes:
- Pre-coated 96-well microplate
- Human MMP2 standard
- Standard dilution buffer
- Biotinylated detection antibody
- Streptavidin-HRP conjugate
- HRP diluent
- Wash buffer
- Chromogen/substrate solution
- Stop solution
- Adhesive plate covers
Storage & Shipping
| Condition | Requirement |
|---|---|
| Shipping | Wet ice |
| Storage Temperature | 2°C to 8°C |
| Product Size | 96 tests |
Ordering Information
| Catalog Number | Description | Format |
|---|---|---|
| KHC3081 | Invitrogen™ Human MMP2 ELISA Kit | 96 Tests |
| KHC3082 | Invitrogen™ Human MMP2 ELISA Kit | 2 × 96 Tests |
Target Information: Human MMP2
Matrix metalloproteinase-2 (MMP2) is a zinc-dependent endopeptidase capable of degrading extracellular matrix components such as collagen, laminin, and fibronectin. It plays an essential role in:
- Tissue remodeling
- Angiogenesis regulation
- Cancer invasion and metastasis
- Inflammatory and connective tissue disorders
Mutations in the MMP2 gene have been linked to rare skeletal syndromes such as Winchester syndrome and NAO syndrome.
Regulatory Status
For Research Use Only. Not for use in diagnostic procedures.
The Invitrogen™ Human MMP2 ELISA Kit (KHC3081) provides a sensitive, validated, and reliable method for quantifying MMP2 levels in serum, plasma, and cell culture samples. With excellent precision, a wide assay range, and standardized sandwich ELISA performance, it is an essential tool for researchers investigating extracellular matrix remodeling, angiogenesis, cancer progression, and biomarker discovery.
- Pack Size: 2 x 96 Tests Plate 96 Tests Plate