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Thermo Scientific™ Tween 20 Washing Buffer, 0.5% in PBS (10X)
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Concentrated PBS-T Wash Solution for Western Blotting and ELISA Applications
Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Tween 20 Washing Buffer, 0.5% in PBS (10X) is a high-quality concentrated wash buffer designed for routine and sensitive immunodetection workflows. Commonly referred to as PBS-T, this buffer combines phosphate-buffered saline with the nonionic detergent Tween 20, providing excellent washing performance while maintaining stable pH conditions.
It is widely used as a wash solution for Western blot membranes, ELISA microtiter plate wells, and other antibody-based assay systems. The optimized formulation effectively removes unbound proteins, antibodies, and excess reagents, reducing background signal and improving assay clarity.
Supplied as a convenient 10X concentrate, this solution supports efficient preparation of working-strength wash buffer for daily laboratory use.
Key Features and Benefits
Optimized for Western Blot and ELISA Washing
PBS-T with Tween 20 is a standard wash buffer in immunoassays, helping improve signal-to-noise ratio by reducing nonspecific binding.
Effective Removal of Excess Material
The detergent component enhances washing efficiency by removing residual antibodies, proteins, and contaminants from membranes and plate surfaces.
Stable pH and Ionic Strength
Phosphate-buffered saline provides consistent buffering capacity and salt composition, supporting reliable assay performance.
Convenient Concentrated Format
Delivered as a 10X solution, allowing easy dilution to 1X working concentration and reducing preparation time.
Suitable for Biomedical and Laboratory Research
Used extensively in diagnostic development, protein analysis, and general laboratory detergent applications.
Applications
Thermo Scientific™ Tween 20 Washing Buffer is commonly used for:
- Western blot membrane washing steps
- ELISA microplate washing
- Immunoassay protocols requiring low background
- Removal of unbound antibodies and reagents
- General laboratory detergent and biomedical research workflows
Product Specifications
| Parameter | Description |
|---|---|
| Product Name | Tween 20 Washing Buffer |
| Composition | 0.5% Tween 20 in PBS |
| Concentration | 10X Stock Solution |
| Physical Form | Liquid |
| Chemical Type | Buffer |
| Recommended Storage | Keep Refrigerated |
| Primary Use | Western blot and ELISA wash solution |
| Unit Size | Each |
Ordering Information
| Catalog Number | Pack Size | Alternate Name |
|---|---|---|
| J63596.AP | 500 mL | J63596-AP |
| J63596.K2 | 1 L | J63596-K2 |
Thermo Scientific™ Tween 20 Washing Buffer, 0.5% in PBS (10X), is a reliable and convenient concentrated wash solution for Western blotting, ELISA, and other immunoassay workflows. Its optimized PBS-detergent formulation ensures effective removal of excess materials, reduced nonspecific binding, and consistent assay performance. An essential reagent for molecular biology and biomedical laboratories requiring reproducible immunodetection results.
- Pack Size: 1L 500 mL