Invitrogen™ JC-9 Dye (Mitochondrial Membrane Potential Probe), 5mg

The Invitrogen™ JC-9 Dye is a ratiometric mitochondrial membrane potential probe designed for live cell imaging of mitochondrial function. Unlike JC-1, the JC-9 dye exhibits green fluorescence that is independent of membrane potential and increases in red fluorescence at hyperpolarized membrane potentials. This behavior makes JC-9 a valuable tool for studying mitochondrial membrane potential changes during processes such as apoptosis, cell stress, and metabolic shifts in live cells.

Since JC-9 is optimized for live cell analysis, it provides a non-invasive and sensitive method for mitochondrial membrane potential studies. It is particularly useful for assessing mitochondrial health, detecting changes in membrane potential, and conducting mitochondrial stress assays.

Key Features:

1. Ratiometric Fluorescence:
   • Exhibits green fluorescence at baseline and increased red fluorescence at hyperpolarized membrane potentials, providing a ratiometric reading of the mitochondrial membrane potential (ΔΨm).
2. Mitochondrial Membrane Potential Indicator:
   • Ideal for quantifying changes in mitochondrial function, particularly in apoptotic or stress-induced cells, allowing for early detection of mitochondrial dysfunction.
3. Live Cell Compatibility:
   • Specifically designed for use with live cells that have active mitochondria. JC-9 is not compatible with fixed cells, making it suitable for real-time studies.
4. Fluorescent Detection:
   • Can be used with fluorescence microscopes and flow cytometers, enabling non-invasive monitoring and analysis of mitochondrial health and cellular processes.
5. Convenient Storage and Handling:
   • Room temperature storage and protection from light ensure the stability and longevity of the dye.

Specifications:

Applications:

1. Mitochondrial Health Monitoring:
   • JC-9 is used to study the functional status of mitochondria in various cell types, helping researchers understand mitochondrial health and dysfunction.
2. Apoptosis and Cell Death Studies:
   • The dye is essential for monitoring mitochondrial membrane potential changes during apoptosis and other programmed cell death processes.
3. Metabolic Studies:
   • JC-9 can be used to assess metabolic shifts in cells, especially those that involve changes in mitochondrial activity and energy production.
4. Cellular Stress and Toxicity:
   • The probe is useful for identifying mitochondrial dysfunction in response to cellular stress or toxic compounds.
5. Drug Development and Screening:
   • JC-9 can be employed to test the effects of potential pharmaceutical agents on mitochondrial function, aiding in drug discovery and toxicology testing.

Storage and Handling:

• Store at room temperature and protect from light to maintain the integrity and effectiveness of the dye for accurate analysis.

Invitrogen™ JC-9 Dye is a highly effective and non-invasive tool for studying mitochondrial membrane potential in live cells. Its ratiometric fluorescence offers researchers a reliable way to measure mitochondrial health, detect apoptosis, and monitor cellular stress, making it invaluable for cell biology, drug development, and metabolic research.