Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells, 115 mL for 50 preps

The Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells provides an easy and versatile microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in approximately 40 minutes. This kit offers a reagent-based isolation method that simplifies and speeds up the traditionally labor-intensive process of mitochondrial isolation, allowing for multiple samples to be processed simultaneously.

The isolation method is highly flexible and can be optimized for purity or yield by following the provided guidelines. It’s designed for researchers looking to isolate mitochondria quickly and effectively for use in a variety of downstream applications, such as apoptosis studies, signal transduction, metabolic research, and mitochondrial proteomics.

Key Features:

1. Fast and Efficient:
   • Isolate intact mitochondria from 20 million cultured cells in just 40 minutes (post-cell harvest). This time-efficient process is ideal for labs that require high throughput and reliable mitochondrial isolation.
2. Flexible Method:
   • The kit offers a reagent-based method for simultaneous processing of several samples, or an optimized Dounce homogenization method for the highest yield, giving flexibility depending on experimental needs.
3. Optimizable:
   • Guidelines provided allow researchers to optimize purity vs. yield for their specific applications, helping to balance efficiency and quality of isolated mitochondria.
4. Benchtop-Easy:
   • The entire isolation process is conducted in a microcentrifuge tube, requiring minimal equipment, which is ideal for small-scale laboratories or those with limited space.
5. Compatible with Downstream Applications:
   • Isolated mitochondria can be lysed with detergent or processed for a wide variety of applications, including 2D electrophoresis, apoptosis studies, and metabolic profiling.
6. Non-Mechanical Reagent-Based Method:
   • Unlike traditional methods, which involve single-sample processing and mechanical homogenization, the reagent-based method allows for the parallel processing of multiple samples, making it easier and faster to obtain reliable results.
7. Optimized Dounce Homogenization Option:
   • The kit includes an optimized Dounce homogenization procedure that results in two-fold greater mitochondrial recovery compared to the reagent-based method, providing an alternative for researchers prioritizing maximum yield.
8. Quick and Easy Isolation:
   • Differential centrifugation is used for separating mitochondrial and cytosolic fractions, allowing the process to be completed in approximately 40 minutes.

Applications:

1. Mitochondrial Research:
   • Used to isolate mitochondria for studies involving apoptosis, signal transduction, and metabolic studies.
2. Proteomics and Genomics:
   • Facilitates mitochondrial proteomics studies by providing pure mitochondria for protein extraction and analysis.
3. Environmental and Pharmacological Testing:
   • Provides high-quality mitochondria for testing how different chemicals or environmental factors affect mitochondrial function.
4. Cell Biology:
   • Used in basic cell biology research to explore the role of mitochondria in various cellular processes such as energy production, cellular respiration, and oxidative stress.
5. Drug Development:
   • Helps isolate mitochondria from cells in pharmaceutical research, allowing for drug testing and the study of mitochondrial dysfunction.

Specifications:

What’s Included in the Kit:

• Reagents and buffers needed for mitochondria isolation.
• Measuring platform and necessary accessories for processing.
• Detailed protocol for optimizing the isolation and use of mitochondria for research applications.

Storage:

• Store the kit at 4°C upon receipt and protect from moisture to ensure reagent stability and reliability.

The Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells is a highly efficient, versatile solution for isolating intact mitochondria from cultured mammalian cells. Whether you require high purity or maximum yield, this kit provides optimized workflows for various downstream applications such as apoptosis studies, signal transduction, and mitochondrial proteomics.