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Gibco™ StemPro™ Accutase™ Cell Dissociation Reagent, 100mL
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Specifications:
| Application | Cell culture | ||
| Storage Temperature | -20°C | ||
| Product Type | Culture Medium | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
The Gibco™ StemPro™ Accutase™ Cell Dissociation Reagent is a gentle, serum-free, animal-origin–free enzymatic solution designed for efficient detachment of a wide variety of adherent cell types while maintaining high cell viability. Unlike animal-derived enzymes such as trypsin, StemPro™ Accutase™ preserves surface epitopes and protein integrity, ensuring accurate downstream analysis of cell surface markers and cell functionality.
StemPro™ Accutase™ is composed of a balanced mixture of proteolytic and collagenolytic enzymes optimized for routine cell passage, virus growth assays, tumor cell migration studies, and flow cytometry. Its ability to dissociate cells with minimal damage makes it particularly suited for primary cells and stem cells, including sensitive human embryonic, mesenchymal, and neural stem cells.
Key Features
- Gentle and Effective – Maintains high cell viability compared to trypsin.
- Serum-Free, Animal-Origin–Free – Reduces variability and risk of contamination.
- Broad Applicability – Effective with stem cells, primary cells, and established cell lines.
- Optimized for Sensitive Applications – Suitable for cell surface marker analysis, virus assays, migration assays, and flow cytometry.
- Ready-to-Use – Convenient frozen liquid format.
Tested Cell Types
- Human embryonic stem cells (hESCs)
- Human mesenchymal stem cells (hMSCs)
- Human neural stem cells (hNSCs)
- Primary cells (fibroblasts, macrophages, keratinocytes, endothelial cells, hepatocytes)
- Established cell lines: CHO, BHK, and 293 cells
Applications
- Routine cell passage and expansion.
- Bioreactor and large-scale culture applications.
- Cell surface marker analysis (flow cytometry).
- Tumor cell migration and proliferation assays.
- Virus growth assays.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Cell Dissociation Reagent |
| Catalog Number | A1110501 |
| Quantity | 100 mL |
| Form | Frozen liquid |
| Classification | Serum-free, animal-origin–free |
| Culture Type | Adherent cells |
| Active Components | Proteolytic and collagenolytic enzymes |
| pH Range | 6–8 |
| Storage Conditions | –5 to –20 °C; protect from light |
| Shelf Life | 24 months |
| Shipping Condition | Dry ice |
Unique Advantages
- Provides a gentler alternative to trypsin, preserving cell integrity.
- Ensures high viability of delicate cells, such as stem cells and primary cultures.
- Eliminates serum and animal-derived components for reproducibility in sensitive workflows.
- Reduces variability and improves consistency in regulated or translational research applications.
The Gibco™ StemPro™ Accutase™ Cell Dissociation Reagent is a versatile, gentle, and serum-free solution for detaching adherent cells while preserving their viability and functional integrity. Designed for use with sensitive primary and stem cells, as well as common cell lines, it is the reagent of choice for routine passaging, flow cytometry, virus assays, and advanced cell biology applications.