Gibco™ KnockOut™ DMEM/F-12

Gibco™ KnockOut™ DMEM/F-12 is a specialized low-osmolality basal medium designed for the growth and maintenance of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). It provides an optimized environment that supports pluripotency and differentiation potential, enabling reliable and reproducible results in stem cell research.

Key Features

1. Low Osmolality:
   • Provides optimal physiological conditions specifically tailored for the growth of stem cells.
2. Omission of L-Glutamine and HEPES Buffer:
   • Allows for customized supplementation to meet specific experimental requirements.
3. Contains Phenol Red:
   • Acts as a pH indicator to monitor medium stability and culture conditions.
4. Manufacturing Quality:
   • Produced under cGMP standards for medical devices, complying with 21 CFR Part 820 and ISO 13485 standards, ensuring high consistency and quality.
5. Convenient Format:
   • Pre-sterilized liquid medium ready for supplementation and use.

Applications

• Stem Cell Culture:
  • Supports the maintenance and expansion of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) while maintaining their pluripotency.
• Differentiation Studies:
  • Serves as a basal medium for protocols requiring the directed differentiation of stem cells into specific lineages.
• Organoid Research:
  • Provides an optimized base for culturing organoids derived from pluripotent stem cells.
• Drug Screening:
  • Ensures consistent growth conditions for stem cells in high-throughput screening applications.
• Tissue Engineering:
  • Used in protocols involving stem cell-based tissue generation and regenerative medicine research.

Specifications

Preparation and Supplementation

1. Add L-Glutamine:
   • Supplement with 200 mM L-Glutamine or GlutaMAX™ to support protein synthesis and cellular metabolism.
2. Buffering:
   • If required, add HEPES buffer for maintaining stable pH in CO₂-independent culture systems.
3. Fetal Bovine Serum (FBS):
   • Supplement with KnockOut Serum Replacement (KSR) or FBS for enhanced stem cell performance.
4. Growth Factors:
   • Add specific growth factors like FGF, Activin A, or BMP4, depending on the application or differentiation protocol.

Advantages

• Customizable: Allows tailored supplementation for specific experimental needs.
• Optimized for Stem Cells: Ensures robust growth and maintenance of stem cell pluripotency.
• Reproducibility: High-quality manufacturing ensures batch-to-batch consistency for reliable experimental outcomes.
• Versatility: Suitable for a variety of downstream applications, including differentiation and drug screening.

Storage and Stability

• Store at 2° to 8°C.
• Protect from light to maintain medium stability and effectiveness.
• Ensure sterility by working in a clean and controlled environment during supplementation.

This specialized medium is the gold standard for stem cell culture, offering researchers a reliable and adaptable foundation for advancing discoveries in regenerative medicine, developmental biology, and disease modeling.