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Thermo Scientific™ Pierce™ Primary Cardiomyocyte Isolation Kit
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Specifications:
| Application | Protein Purification | ||
| Storage Temperature | Upon receipt store Product 88281X at 4°C and Product 88281Y at -20°C. | ||
| Product Type | Molecular Biology Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Pierce™ Primary Cardiomyocyte Isolation Kit enables fast, reproducible isolation of viable, functional primary cardiomyocytes from neonatal rat and mouse heart tissue. Designed with tissue-specific enzymatic reagents, optimized culture conditions, and a streamlined protocol, this kit significantly improves both the yield and viability of isolated cells compared to traditional or homemade methods.
By eliminating the need for prolonged enzymatic digestions and reducing the number of manual steps, the entire isolation procedure can be completed in under 2 hours. The resulting cardiomyocytes exhibit robust beating activity, express relevant protein markers, and are ideal for a wide range of biochemical, functional, and imaging assays.
Key Features and Benefits
- Rapid Protocol – Isolation and seeding completed in under 2 hours
- High Viability and Yield – 1.5- to 4-fold higher yield vs. manual or competitor methods
- Validated Functional Output – Isolated cardiomyocytes exhibit contractility and express cardiac markers
- Optimized Enzymatic Digestion – Includes tissue-specific enzymes (papain and thermolysin) to prevent over-digestion
- Complete Culture System – Includes DMEM, HBSS, and a cardiomyocyte-specific growth supplement
Kit Components
- Cardiomyocyte Isolation Enzyme 1 (with papain)
- Cardiomyocyte Isolation Enzyme 2 (with thermolysin, 20X)
- Hanks' Balanced Salt Solution (HBSS)
- DMEM for Primary Cell Isolation
- Cardiomyocyte Growth Supplement
Applications
- Cardiomyocyte differentiation and maturation studies
- Immunohistochemistry (IHC)
- Functional analysis (e.g., contractility, toxicity assays)
- Preclinical drug testing and predictive disease modeling
- Studies on cardiac ischaemia, hypoxia, and metabolic stress
Specifications
| Attribute | Details |
|---|---|
| Cell Type | Primary Cardiomyocytes |
| Target Species | Rat, Mouse |
| Assay Compatibility | IHC, biochemical assays, functional assays |
| Yield Advantage | 1.5x–4x more cells than conventional methods |
| Time to Completion | < 2 hours |
| Format | Complete Kit (1 L) |
| Product Line | Thermo Scientific™ Pierce™ |
| Storage | Store Component 88281X at 4°C; 88281Y at –20°C |
| Shipping | 88281X: Ice packs; 88281Y: Dry ice |
Related Products
- Cardiomyocyte Isolation Enzyme 1 (with papain)
- Cardiomyocyte Isolation Enzyme 2 (20X, with thermolysin)
- DMEM for Pierce™ Cell Isolation Kits
- HBSS without Ca²⁺/Mg²⁺ for Tissue Dissociation
The Thermo Scientific™ Pierce™ Primary Cardiomyocyte Isolation Kit provides a robust, validated solution for the isolation and short-term culture of functional cardiomyocytes from neonatal hearts. Whether you're conducting cardiac toxicity testing, studying contractility, or performing biochemical assays, this kit offers a time-saving, high-performance system with consistent results across a range of primary cell biology workflows.