Thermo Scientific™ Pierce™ Primary Cardiomyocyte Isolation Kit

The Thermo Scientific™ Pierce™ Primary Cardiomyocyte Isolation Kit is an optimized solution for the rapid, high-yield isolation and culture of primary cardiomyocytes from neonatal mouse and rat hearts. This kit contains validated enzymatic dissociation reagents and culture media to ensure high cell viability, purity, and functionality.

Unlike traditional cardiac cell isolation methods, which require multiple incubations and long digestion times (>16 hours), this kit enables efficient cardiomyocyte isolation in under 2 hours, yielding up to 4-fold higher viable cell counts compared to other commercial kits.

Isolated cardiomyocytes exhibit contractile function (beating activity) and express validated biochemical markers, making them ideal for functional assays, disease modeling, and preclinical drug discovery.

Key Features & Benefits

1. High-Yield, High-Viability Cardiomyocyte Isolation
   • Provides 1.5- to 4-fold greater yield compared to DIY methods.
   • Ensures higher viability and functional activity of isolated cells.
2. Rapid & Efficient Cell Isolation in Under 2 Hours
   • Saves time compared to traditional overnight digestion methods.
   • Prevents overdigestion and loss of viable cardiomyocytes.
3. Validated Enzymatic Dissociation for Optimal Purity
   • Uses tissue-specific dissociation enzymes (papain & thermolysin) for gentle digestion.
   • Reduces contaminating fibroblasts, increasing cardiomyocyte purity.
4. Complete Kit with Ready-to-Use Reagents
   • Includes all required buffers, media, and growth supplements.
   • Optimized for consistent and reproducible primary cardiomyocyte isolation.
5. Ideal for Functional & Biochemical Assays
   • Isolated cardiomyocytes retain electrophysiological and contractile activity.
   • Suitable for ischemia, hypoxia, and cardiotoxicity research.

Product Specifications

Kit Components

• Cardiomyocyte Isolation Enzyme 1 (with papain)
• Cardiomyocyte Isolation Enzyme 2 (with thermolysin) (20X)
• Hanks' Balanced Salt Solution (HBSS) (No Ca²⁺, Mg²⁺)
• DMEM for Primary Cell Isolation
• Cardiomyocyte Growth Supplement

Use Cases & Applications

1. Cardiomyocyte Differentiation & Culture

• Enhances Cardiomyocyte Yield & Purity
  • Optimized enzymatic digestion ensures selective isolation of cardiomyocytes.
  • Reduces fibroblast contamination, improving experimental reproducibility.
• Supports Long-Term Culture of Cardiomyocytes
  • Cultures can be maintained for up to 7 days with the included media and supplements.
  • Allows for electrophysiological characterization of cardiomyocytes.

2. Immunohistochemistry (IHC) & Functional Assays

• Cardiac Protein Marker Expression Analysis
  • Isolated cells express cardiomyocyte-specific biochemical markers.
  • Suitable for immunostaining and fluorescence-based imaging.
• Electrophysiological & Contraction Studies
  • Isolated cardiomyocytes exhibit validated beating activity.
  • Ideal for calcium imaging and electrophysiology studies.

3. Preclinical Drug Discovery & Toxicology

• In Vitro Cardiomyocyte Models for Drug Testing
  • Enables testing of cardiotoxic compounds and arrhythmia-inducing agents.
  • Provides a predictive system for pharmacological evaluations.
• Disease Modeling for Cardiac Conditions
  • Allows investigation of ischemia, hypoxia, and metabolic disorders in cardiomyocytes.
  • Used for genetic and epigenetic studies of cardiovascular diseases.

4. Cardiovascular Regenerative Medicine Research

• Modeling Cardiac Regeneration Pathways
  • Supports stem cell differentiation into functional cardiomyocytes.
  • Used in tissue engineering and heart failure studies.
• Evaluation of Growth Factors & Cytokine Signaling
  • Investigates the role of VEGF, TGF-β, and Wnt signaling in cardiomyocyte development.
  • Helps in identifying novel regenerative medicine targets.

Instructions for Use

Cell Isolation Protocol:

1. Tissue Preparation & Digestion:
   • Wash neonatal heart tissue in HBSS (without Ca²⁺, Mg²⁺) before enzymatic digestion.
   • Digest tissue using Cardiomyocyte Isolation Enzymes 1 & 2.
2. Cell Washing & Collection:
   • Centrifuge and resuspend isolated cells in HBSS (without Ca²⁺, Mg²⁺).
   • Wash cells twice to remove enzyme contaminants.
3. Cell Culture & Assay Preparation:
   • Resuspend washed cells in DMEM supplemented with Cardiomyocyte Growth Supplement.
   • Plate cells in culture vessels for further differentiation or functional assays.

Storage & Handling Instructions

• Storage Conditions:
  • Store Product 88281X at 4°C and Product 88281Y at -20°C.
  • Avoid repeated freeze-thaw cycles to maintain reagent stability.
• Handling Precautions:
  • Use aseptic techniques when handling cells and media.
  • Work in a biosafety cabinet (BSC) to prevent contamination.
• Disposal Guidelines:
  • Dispose of used buffers and reagents according to laboratory biosafety regulations.
  • If used with biohazardous samples, autoclave before disposal.

Why Choose Thermo Scientific™ Pierce™ Primary Cardiomyocyte Isolation Kit?

✅ High-yield, high-viability cardiomyocyte isolation in under 2 hours

✅ 1.5- to 4-fold increase in cell yield compared to traditional methods

✅ Validated enzymatic digestion with optimized growth supplements

✅ Cardiomyocytes express protein markers and exhibit functional beating activity

✅ Reliable for preclinical drug discovery, toxicology studies, and disease modeling

Thermo Scientific™ Pierce™ Primary Cardiomyocyte Isolation Kit is the gold standard for high-purity, high-viability primary cardiomyocyte isolation, ensuring consistent and reproducible results for cardiac research applications.