VWR Phenol : chloroform : iso-amyl alcohol (25:24:1) (v/v/v) for biotechnology

Phenol / Chloroform #2- A 25:24:1 mixture of phenol, chloroform, and isoamyl alcohol useful for preparing RNA with maximal recovery of poly (A+) RNA for subsequent mRNA purification and generation of cDNA.

• Manufactured Using Exceptionally High Purity Phenol that is Double Distilled & Packaged Under Nitrogen
• Liquid Phenols Offer a Safer, Easier Alternative to Crystalline Phenol
• Optimized pH for the Purification of RNA
• Long Term Stability > One Year

For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation. VWR’s phenol products are manufactured from the purest crystalline phenol to ensure optimal results.