QuickTiter™ Hepatitis B "e" Antigen (HBeAg) ELISA Kit

The QuickTiter™ Hepatitis B "e" Antigen ELISA Kit is a highly sensitive and specific sandwich ELISA designed for the detection and quantitation of Hepatitis B "e" antigen (HBeAg). With a sensitivity as low as 150 pg/mL, this kit provides an efficient and accurate solution for measuring HBeAg levels in purified virus samples or unpurified viral supernatants.

Product Highlights:

1. High Sensitivity:
   • Detects HBeAg levels as low as 150 pg/mL, making it ideal for low-abundance antigen detection.
2. Quantitative Results:
   • Provides a standard curve for accurate HBeAg quantitation in unknown samples.
3. Comprehensive Kit Components:
   • Reagents sufficient for 96 wells, including standards, unknown samples, and controls.
4. Specific Detection Methodology:
   • Utilizes a sandwich ELISA format with a FITC-conjugated detection antibody and HRP-conjugated secondary antibody for enhanced specificity and signal amplification.

Principle of the Assay:

The assay employs a sandwich ELISA methodology for detecting HBeAg:

1. Capture Phase:
   • An anti-HBeAg antibody pre-coated on the ELISA plate captures HBeAg present in the sample.
2. Detection Phase:
   • A FITC-conjugated anti-HBeAg antibody binds to the captured antigen-antibody complex.
3. Signal Development:
   • An HRP-conjugated anti-FITC antibody binds to the FITC label, forming a tertiary complex.
4. Signal Quantification:
   • TMB substrate is added, producing a colorimetric signal directly proportional to the HBeAg concentration.
5. Measurement:
   • The optical density (OD) is measured at 450 nm, and HBeAg levels in unknown samples are calculated using a standard curve.

Applications and Use Cases:

1. Clinical Research:

• HBV Infection Monitoring:
  • Detect and quantify HBeAg levels to evaluate active HBV infection and infectivity.
• Disease Progression Studies:
  • Monitor HBeAg clearance during HBV treatment or recovery phases.

2. Vaccine and Therapeutic Development:

• Vaccine Efficacy Studies:
  • Measure HBeAg levels to evaluate vaccine-induced immune responses.
• Drug Development:
  • Assess the impact of antiviral drugs targeting HBV replication.

3. Epidemiology and Public Health:

• HBV Prevalence Studies:
  • Screen populations for active HBV infection and assess viral antigen load.
• Outbreak Investigations:
  • Analyze viral samples during HBV outbreak scenarios.

4. Virology Research:

• HBV Pathogenesis:
  • Study the role of HBeAg in HBV infection and immune evasion mechanisms.
• Virus Propagation Studies:
  • Quantify HBeAg in viral supernatants to assess viral replication efficiency.

Kit Components:

• Pre-Coated 96-Well Plate: Anti-HBeAg antibody coated for specific antigen capture.
• HBeAg Standards: For generating a standard curve for quantitation.
• FITC-Conjugated Anti-HBeAg Antibody: Specific for HBeAg detection.
• HRP-Conjugated Anti-FITC Antibody: Amplifies the detection signal.
• Sample Diluent: Optimizes sample preparation.
• Wash Buffer: Removes unbound components.
• TMB Substrate: Produces a colorimetric reaction proportional to antigen levels.
• Stop Solution: Stabilizes the color change for accurate OD readings.
• Instruction Manual: Provides a step-by-step protocol for accurate assay execution.

Performance Specifications:

• Sensitivity: 150 pg/mL
• Dynamic Range: Based on the provided HBeAg standards.
• Sample Compatibility: Purified virus samples or unpurified viral supernatants.

Advantages of the QuickTiter™ HBeAg ELISA Kit:

1. Ease of Use:
   • Ready-to-use reagents and pre-coated plates streamline the assay process.
2. Specific and Accurate:
   • Highly specific sandwich ELISA design ensures reliable HBeAg detection with minimal cross-reactivity.
3. Rapid Results:
   • Generates quantitative results efficiently, facilitating timely research or clinical decision-making.
4. Wide Applications:
   • Supports diverse research and clinical objectives, including virology, immunology, and public health.

Sample Preparation Recommendations:

1. Purified Virus Samples:
   • Use directly or dilute as required based on expected HBeAg concentration.
2. Viral Supernatants:
   • Clarify by centrifugation to remove debris before testing.

Storage Conditions:

• Store all reagents as per the manufacturer's instructions, typically at 2–8°C.
• Avoid repeated freeze-thaw cycles to maintain reagent integrity.

The QuickTiter™ Hepatitis B "e" Antigen ELISA Kit is an indispensable tool for researchers and clinicians studying HBV infection, immune responses, and viral dynamics. Its high sensitivity, specificity, and comprehensive kit design make it ideal for detecting and quantifying HBeAg in various experimental and clinical settings.