0
Varioskan™ LUX Multimode Microplate Reader (Absorbance, Fluorescence Intensity, Luminescence, with Gas Control Module)
Catalog No :
CAS Number :
Brand :
In Stock
Reading Options: Top and bottom
No. of dispensers: None
Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Ambient | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL00GD0) is an advanced, modular, and upgradeable instrument designed to deliver superior flexibility and precision in absorbance, fluorescence intensity, and luminescence detection.
Built for bioscience and life science researchers, the Varioskan LUX provides outstanding performance for a wide range of applications, including cell-based assays, enzyme kinetics, nucleic acid quantification, and luminescence-based reporter assays.
This model comes equipped with an integrated Gas Control Module for precise regulation of CO₂ (0.1–15%) and O₂ (1–21%), allowing seamless operation in cellular and live-cell assays. With automatic calibration, dynamic range selection, and smart safety controls, it combines speed, reliability, and convenience in a compact footprint.
Key Features and Benefits
1. Comprehensive Multimode Detection
- Absorbance (UV-Vis, 200–1000 nm), Fluorescence Intensity, and Luminescence detection.
- Top and bottom reading optics ensure flexibility for solution-based and cell-based assays.
- Dual monochromator optical design offers full spectral control for excitation and emission.
- Compatible with 6–1536 well plates and μDrop plates for small-volume applications.
2. Integrated Gas Control for Cell-Based Assays
- Built-in Gas Module allows adjustment of CO₂ (0.1–15%) and O₂ (1–21%) for physiological conditions.
- Ideal for cell viability, proliferation, and metabolic activity assays.
- Provides stable and controlled assay environments for reliable results.
3. High Sensitivity and Dynamic Range
-
Fluorescence Sensitivity:
- Top reading: <0.4 fmol fluorescein/well.
- Luminescence Sensitivity: <7 amol ATP/well (white 384-well plate).
- Dynamic Range: Fluorescence >6 decades (top), Luminescence >7 decades.
- Photometric Accuracy: ±2% (200–399 nm), ±1% (400–1000 nm).
- Precision: SD <0.001 Abs or CV <0.5% at 450 nm.
4. Intelligent Automation and Workflow Optimization
- Automatic dynamic range selection ensures accurate detection for each well.
- Smart safety controls alert users to setup or operational errors before measurement.
- Automated self-calibration and instrument diagnostics maintain performance accuracy.
- Spectral scanning, endpoint, kinetic, and multipoint modes for comprehensive assay flexibility.
- Orbital shaking and temperature control (ambient +4°C to 45°C) promote consistent reaction conditions.
5. Powerful SkanIt™ Software Integration
- Thermo Scientific™ SkanIt™ Software (Research Edition) included for instrument control and analysis.
- License-free installation for multi-user environments.
- Virtual Pipetting Tool simplifies assay setup and sample mapping.
- Real-time kinetic and spectral data visualization.
- Automatic data backup prevents data loss during interruptions.
- Built-in data analysis tools: blank subtraction, curve fitting, kinetic modeling, and dose-response analysis.
- Flexible export options: *.xlsx, *.pdf, *.xml, *.txt; automatic email delivery post-run.
6. Certified and Validated Performance
- HTRF™ certified (Cisbio Bioassays).
- DLReady™ validated (Promega).
- Designed for reliable assay compatibility across major fluorescence and luminescence platforms.
Technical Specifications
| Parameter | Specification |
|---|---|
| Measurement Technologies | Absorbance, Fluorescence Intensity, Luminescence |
| Reading Options | Top and Bottom |
| Wavelength Range | Absorbance: 200–1000 nm; Fluorescence Excitation: 200–822 nm; Emission: 270–840 nm; Luminescence: 360–670 nm |
| Measurement Speed | 15 s (96-well), 45 s (384-well), 135 s (1536-well) |
| Dynamic Range | Fluorescence Intensity: >6 decades; Luminescence: >7 decades |
| Sensitivity | FI (top): <0.4 fmol fluorescein/well; Luminescence: <7 amol ATP/well |
| Accuracy (Photometric) | ±2% (200–399 nm, 0–2 Abs); ±1% (400–1000 nm, 0–3 Abs) |
| Precision | SD <0.001 Abs or CV <0.5% at 450 nm |
| Linearity | ±2% (96-well 0–4 Abs; 384-well 0–3 Abs at 450 nm) |
| Spectral Scanning Speed | <2.2 s/well (400–500 nm, 2 nm step) |
| Gas Control Range | CO₂: 0.1–15%; O₂: 1–21% |
| Light Source | Xenon Flash Lamp |
| Detector Type | Photomultiplier Tube (PMT) |
| Incubation Temperature | Ambient +4°C to 45°C |
| Shaking | Orbital |
| Interface | PC Software (SkanIt™ Software) |
| Software Languages | English, French, German, Spanish, Portuguese, Italian, Japanese, Chinese |
| Dimensions (W × D × H) | 53 × 58 × 51 cm (21 × 23 × 20 in) |
| Weight | 57 kg (125 lb) |
| Power Requirements | 100–240 V, 50/60 Hz |
| Plate Formats | 6–1536 well plates (Absorbance: up to 384 wells), μDrop plates |
| Software Edition | Research Edition |
Applications
- Cell-Based Assays: Reporter gene, proliferation, cytotoxicity, and metabolic studies under controlled gas conditions.
- Nucleic Acid and Protein Quantification: DNA/RNA and protein assays using absorbance and fluorescence.
- Enzyme Kinetics: Kinetic monitoring of enzyme activity and inhibition.
- Luminescence Assays: ATP quantification, luciferase expression, and cell viability testing.
- Fluorescence Assays: FRET, GFP, and probe-based applications.
- Drug Discovery and Screening: High-throughput luminescent and fluorescent analysis.
- Spectral Scanning: Optimization of excitation and emission wavelengths for assay development.
Unique Advantages
- Integrates absorbance, fluorescence, and luminescence in a single platform.
- Built-in Gas Control Module for cell-based and physiological assays.
- Automatic calibration and dynamic range adjustment enhance reproducibility.
- HTRF™ and DLReady™ certifications ensure validated performance.
- Modular, upgradeable design accommodates future assay expansion.
- SkanIt™ Software delivers comprehensive data analysis, traceability, and user management.
The Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader (VLBL00GD0) combines three core detection technologies—absorbance, fluorescence, and luminescence—with a built-in Gas Control Module, ensuring precision for both standard biochemical and cell-based assays.
With top and bottom reading optics, spectral scanning, and SkanIt™ Software integration, the Varioskan LUX simplifies data acquisition and analysis while maintaining accuracy and reproducibility across applications.
A validated, modular, and intelligent system, the Varioskan LUX empowers research laboratories with unmatched versatility, automation, and confidence in every experiment.