Virplex® Mycobacterium Real-Time PCR Kit, 5-Plex

​The Virplex® MYCOBACTERIUM Real-Time PCR Kit is a multiplex PCR assay designed for the qualitative detection of nucleic acids from Mycobacterium tuberculosis complex (MTBC), Mycobacterium avium complex (MAC), Mycobacterium abscessus complex (MABSC), and other non-tuberculous mycobacteria (NTM) in human sputum samples.

This high-performance molecular assay enables laboratories to rapidly differentiate tuberculous and non-tuberculous mycobacterial infections, ensuring faster and more accurate research and diagnostic decisions in the field of pulmonary and infectious disease testing.

Key Features

• Comprehensive Mycobacterial Detection – Detects MTBC, MAC, MABSC, and other NTM species.
• Multiplex Format (5 Targets) – All-in-one detection using a single reaction tube per sample.
• Built-in Endogenous Control – Monitors sample quality, DNA integrity, and amplification efficiency.
• High Sensitivity & Specificity – Optimized for sputum samples containing mycobacterial pathogens.
• Rapid Results – Delivers accurate results in less than 2 hours.
• Lyophilized Master Mix & Positive Control – Enhances reagent stability and minimizes transport costs.
• Instrument Compatibility – Suitable for 5-channel qPCR thermocyclers.
• CE-IVDR Certified – Fully compliant with European in vitro diagnostic standards (IVDR 2017/746).

Kit Contents

• Lyophilized Master Mix (Multiplex format)
• Endogenous Human Control
• Positive Control
• User Manual

(All reagent volumes are scaled according to 24 or 96 test format.)

Technical Specifications

Applications

• Tuberculosis & NTM Research – Differentiation of Mycobacterium tuberculosis from non-tuberculous mycobacteria.
• Pulmonary Infection Studies – Detection of major mycobacterial complexes (MTBC, MAC, MABSC).
• Clinical Microbiology – Identification of mycobacterial infections in respiratory samples.
• Public Health & Surveillance – Monitoring NTM prevalence and TB-like infections.
• Reference & Academic Labs – Advanced research on mycobacterial diversity and pathogenicity.

Ordering Information

The Virplex® MYCOBACTERIUM Real-Time PCR Kit provides a robust, sensitive, and multiplex molecular assay for detecting both tuberculous and non-tuberculous mycobacteria in human sputum samples. With its broad detection range, integrated endogenous control, and CE-IVDR compliance, this kit delivers rapid, reliable, and standardized results ideal for clinical, research, and public health laboratories.