Virplex® MTBC Species Real-Time PCR Kit, 7-Plex, 24 Rxn

​The Virplex® MTBC SPECIES Real-Time PCR Kit is an advanced multiplex real-time RT-PCR assay for the detection and differentiation of species within the Mycobacterium tuberculosis complex (MTBC), including M. tuberculosis, M. africanum, M. microti, M. caprae, M. bovis, M. bovis BCG, and M. canettii.

Designed for use with culture isolates or smear-positive specimens, this assay enables rapid identification and species-level differentiation of Mycobacterium tuberculosis complex organisms, supporting research on tuberculosis (TB) epidemiology, pathogenesis, and drug-resistance studies.

Key Features

• Comprehensive MTBC Coverage – Detects and differentiates M. tuberculosis, M. africanum, M. microti, M. caprae, M. bovis, M. bovis BCG, and M. canettii.
• Multiplex PCR Format – Two reaction tubes per sample for simultaneous species identification.
• Reliable Internal Control – Monitors amplification efficiency, DNA integrity, and potential inhibition.
• Fast Turnaround – Generates results in under 2 hours.
• High Sensitivity & Specificity – Optimized for smear-positive and culture isolates.
• Lyophilized Reagents – Stable master mix and positive control reduce cold-chain requirements.
• Instrument Compatibility – Suitable for 4-channel real-time PCR cyclers.
• Research Use Only (RUO) – For research and reference laboratory use.

Kit Contents (24 Reactions)

• Lyophilized Master Mix (Multiplex Format)
• Internal Control
• Positive Control
• Two-Tube Reaction Setup
• User Manual

Technical Specifications

Applications

• Tuberculosis Research – Detection and differentiation of MTBC species.
• Epidemiological Studies – Surveillance of Mycobacterium species distribution.
• Zoonotic TB Research – Identification of M. bovis, M. caprae, and M. microti strains.
• Vaccine & Strain Differentiation – Distinguishes M. bovis BCG from wild-type M. bovis.
• Public Health & Reference Labs – Supports TB monitoring and comparative genotyping research.

Ordering Information

The Virplex® MTBC SPECIES Real-Time PCR Kit (RTPCR022-LP-R) offers a reliable, multiplex molecular solution for the rapid detection and differentiation of Mycobacterium tuberculosis complex species. Its ability to identify seven clinically and epidemiologically relevant species makes it a powerful research tool for tuberculosis diagnostics, strain tracking, and public health surveillance. With lyophilized reagents and a simple two-tube format, it combines accuracy, speed, and convenience for modern molecular laboratories.