0
VIASURE™ Yellow Fever Real Time PCR Detection Kit
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | qPCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Kit with Various components |
| Product Brand | Certest Biotec | ||
| Product Grade | Molecular Biology | ||
The VIASURE™ Yellow Fever Real Time PCR Detection Kit is a highly sensitive and specific molecular diagnostic assay designed for the qualitative detection of Yellow Fever Virus (YFV) RNA in clinical samples. Utilizing advanced real-time reverse transcription PCR (RT-PCR) technology, this kit enables rapid and reliable identification of viral RNA using fluorescent probe-based detection.
This assay is intended for use as an aid in the diagnosis of Yellow Fever infection, particularly in patients presenting with compatible clinical symptoms and epidemiological risk factors. The kit provides a streamlined workflow, combining RNA amplification and detection in a single step, making it ideal for clinical research and surveillance laboratories.
Key Features
- Highly sensitive and specific RT-PCR detection of Yellow Fever Virus RNA
- Fluorescent probe-based detection system for accurate results
- Includes internal control to validate assay performance
- Room temperature storage and transport (no cold chain required)
- Long shelf life of up to 2 years
- Ready-to-use format with pre-loaded reaction strips/plates
- Compatible with standard real-time PCR instruments
- Available in multiple formats for flexible throughput
Applications
This kit is ideal for:
- Molecular detection of Yellow Fever Virus (YFV)
- Clinical research and epidemiological surveillance
- Outbreak monitoring and public health screening
-
Differentiation from other febrile illnesses such as:
- Dengue
- Zika virus
- West Nile virus
- Malaria and viral hepatitis
Kit Formats & Ordering Information
| Reference | Format | Description |
|---|---|---|
| VS-FEV106L | Low Profile | 6 × 8-well strips |
| VS-FEV106H | High Profile | 6 × 8-well strips |
| VS-FEV112L | Low Profile | 12 × 8-well strips |
| VS-FEV112H | High Profile | 12 × 8-well strips |
| VS-FEV113L | Low Profile | 96-well plate |
| VS-FEV113H | High Profile | 96-well plate |
Kit Components
| Component | Description |
|---|---|
| Yellow Fever Reaction Mix | Enzymes, primers/probes, buffer, dNTPs, stabilizers, internal control (lyophilized) |
| Rehydration Buffer | Solution for reconstitution of reagents |
| Positive Control | Non-infectious synthetic lyophilized cDNA |
| Negative Control | Non-template control |
| RNase/DNase-free Water | For reaction preparation |
| Optical Caps | For sealing wells during PCR cycling |
Technical Specifications
| Parameter | Specification |
|---|---|
| Detection Method | Real-Time RT-PCR |
| Target | Yellow Fever Virus RNA |
| Specimen Type | Clinical samples |
| Detection System | Fluorescent probe-based |
| Storage Temperature | Room temperature |
| Shelf Life | Up to 2 years |
| Format | Lyophilized reagents in strips/plates |
Target Information
Yellow Fever Virus (YFV) is a mosquito-borne RNA virus belonging to the Flaviviridae family. It is transmitted primarily by Aedes aegypti and Haemagogus mosquito species and is endemic in parts of Africa and Latin America.
The disease ranges from a mild febrile illness to a severe hemorrhagic condition with liver failure, jaundice, and high mortality rates. Due to overlapping symptoms with other infectious diseases such as malaria, dengue, and viral hepatitis, accurate laboratory diagnosis is essential.
Real-time PCR provides a rapid, sensitive, and specific method for detecting viral RNA, enabling early diagnosis and improved outbreak control.
Advantages
- Rapid and accurate detection compared to traditional culture methods
- Eliminates need for complex viral isolation techniques
- High sensitivity for early-stage infection detection
- Stable at room temperature – ideal for resource-limited settings
- Complete kit with controls for assay reliability
- Suitable for high-throughput testing environments
The VIASURE™ Yellow Fever Real Time PCR Detection Kit offers a reliable, sensitive, and user-friendly solution for the molecular detection of Yellow Fever Virus. Its room-temperature stability, comprehensive components, and high diagnostic performance make it an excellent choice for clinical research laboratories, public health institutions, and outbreak response programs.
- Reactions: 6 x 8-well strips, low profile (48 Reactions) 6 x 8-well strips, high profile (48 Reactions) 12 x 8-well strips, low profile (96 Reactions) 12 x 8-well strips, high profile (96 Reactions) 96-well plate, low profile (96 Reactions) 96-well plate, High profile (96 Reactions)