VIASURE™ Flu A+B Real Time PCR Detection Kit

The VIASURE Flu A+B Real Time PCR Detection Kit is a highly sensitive and specific molecular diagnostic assay designed for the simultaneous detection and differentiation of Influenza A and Influenza B viruses in respiratory samples. Utilizing advanced real-time RT-PCR technology with fluorescent probe detection, the kit enables rapid and accurate identification of influenza infections.

Optimized for clinical and research laboratories, this assay supports reliable diagnosis when used alongside clinical and epidemiological data. Its room-temperature stability and flexible format options make it a practical solution for laboratories requiring efficient workflows and broad instrument compatibility.

Key Features

• Dual Detection Capability
  Simultaneous identification and differentiation of Influenza A and Influenza B viruses in a single assay.
• High Sensitivity and Specificity
  Real-time RT-PCR with fluorescent probe detection ensures accurate and reliable results.
• Optimized Target Selection
  Detection based on conserved viral gene regions (e.g., M1 gene) for robust identification.
• Flexible Format Options
  Available in multiple strip and plate configurations to suit different laboratory throughput needs.
• Room Temperature Storage
  Simplifies logistics and reduces cold-chain requirements.
• Ready-to-Use Workflow
  Streamlined protocol minimizes preparation time and reduces risk of contamination.

Applications

Clinical Diagnostics (Research Use / Screening Support)

• Detection of Influenza A and B in symptomatic patients
• Support for respiratory infection diagnosis
• Epidemiological surveillance of influenza outbreaks

Public Health & Surveillance

• Monitoring seasonal influenza trends
• Outbreak investigation and control
• Population-level screening programs

Molecular Research

• Viral detection studies
• Influenza strain monitoring
• Respiratory pathogen research

Assay Principle

1. RNA Extraction from respiratory samples
2. Reverse Transcription & Amplification (RT-PCR)
3. Fluorescent Probe Detection specific for Flu A and Flu B
4. Real-Time Data Analysis for qualitative detection

Technical Specifications

Ordering Information

Clinical & Scientific Background

Influenza viruses, members of the Orthomyxoviridae family, are among the leading causes of viral respiratory infections worldwide. Influenza A and B are the most clinically significant types affecting humans, with Influenza A further classified into subtypes based on hemagglutinin (HA) and neuraminidase (NA) proteins, and Influenza B divided into Victoria and Yamagata lineages.

Transmission occurs via:

• Respiratory droplets and aerosols
• Direct and indirect contact

Symptoms typically include:

• Fever and chills
• Cough and sore throat
• Nasal congestion
• Muscle aches and headaches

Due to the high morbidity and risk of complications—especially in elderly and immunocompromised individuals—rapid and accurate detection is essential for effective clinical management and outbreak control.

Unique Advantages

• Multiplex Detection Efficiency
  Reduces testing time and reagent use by detecting both Flu A and B in a single reaction.
• Operational Flexibility
  Multiple formats accommodate low- to high-throughput laboratory settings.
• Logistics-Friendly Storage
  Room temperature stability simplifies distribution and storage, especially in resource-limited settings.
• Reliable Molecular Performance
  High sensitivity ensures detection even at low viral loads.

The VIASURE Flu A+B Real Time PCR Detection Kit is a robust and versatile solution for the molecular detection of influenza viruses. With its high sensitivity, dual-target capability, and flexible format options, it supports efficient and accurate influenza testing across clinical, research, and public health laboratories.