0
VIASURE®E. coli Typing Real Time PCR Detection Kit
Catalog No :
CAS Number :
Brand :
In Stock
(E. coli ETEC + EIEC) + (E. coli EHEC, EPEC & EIEC)
Specifications:
| Application | qPCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | Certest Biotec | ||
| Product Grade | Molecular Biology | ||
The VIASURE E. coli Typing Real Time PCR Detection Kit is a multiplex molecular assay designed for the rapid, specific detection and differentiation of major pathogenic Escherichia coli (E. coli) pathotypes:
- Enterohemorrhagic E. coli (EHEC)
- Shiga toxin-producing E. coli (STEC)
- Enteropathogenic E. coli (EPEC)
- Enterotoxigenic E. coli (ETEC)
- Enteroinvasive E. coli (EIEC)/Shigella
This kit enables detection directly from human stool specimens through Real-Time PCR using specific primers and fluorescent probes. It includes internal controls for monitoring inhibition and ensures reliable results across high-throughput workflows. Ideal for outbreak investigation, clinical research, and public health surveillance.
Key Features & Benefits
- ✅ Multiplex Detection – Simultaneous identification of multiple E. coli pathotypes
- ✅ Ready-to-Use Format – Lyophilized 8-well strips reduce setup time and minimize pipetting error
- ✅ Internal Controls Included – Ensures result validity and detects PCR inhibition
- ✅ Room Temperature Storage – Simplifies shipping and storage logistics
- ✅ Rapid Turnaround Time – Results within hours for timely decision-making
- ✅ High Sensitivity & Specificity – Based on validated real-time PCR technology
- ✅ Positive & Negative Controls Supplied – Essential for result interpretation
- ✅ Compatible with Standard Thermocyclers – Works with most qPCR platforms
Applications
- Detection and typing of diarrheagenic E. coli in stool samples
- Outbreak surveillance of foodborne pathogens
- Differentiation of EHEC, ETEC, EPEC, EIEC, and Shigella spp.
- Research on gastrointestinal infections
- Support for public health and food safety laboratories
Kit Contents
| Component | Description | Color |
|---|---|---|
| EHEC + EPEC + EIEC 8-well strips | Lyophilized enzyme mix with primers, probes, internal control | White |
| ETEC + EIEC 8-well strips | Lyophilized enzyme mix with primers, probes, internal control | White |
| Rehydration Buffer | Solution to reconstitute stabilized reagent | Blue |
| Positive Control | Non-infectious synthetic lyophilized cDNA | Red |
| Negative Control | RNAse/DNAse-free non-template control | Violet |
| RNAse/DNAse-Free Water | Molecular biology-grade water | White |
| Tear-Off Optical Cap Strips | For sealing wells during thermal cycling | Transparent |
Ordering Information
| Ref. Code | Description | Format |
|---|---|---|
| VS-ECT106L | 6 × 8-well strips, low profile | 48 reactions total |
| VS-ECT106H | 6 × 8-well strips, high profile | 48 reactions total |
| VS-ECT112L | 12 × 8-well strips, low profile | 96 reactions total |
| VS-ECT112H | 12 × 8-well strips, high profile | 96 reactions total |
The VIASURE E. coli Typing Real Time PCR Detection Kit is a powerful molecular tool for identifying and differentiating clinically significant E. coli pathotypes in stool specimens. Its rapid results, high specificity, and user-friendly format make it ideal for clinical research, epidemiological surveillance, and outbreak response. The inclusion of internal controls, positive/negative standards, and room temperature stability supports consistent, accurate testing in both field and lab-based settings.
- Reactions: 6 x 8-well strips, low profile (48 Reactions) 6 x 8-well strips, high profile (48 Reactions) 12 x 8-well strips, low profile (96 Reactions) 12 x 8-well strips, high profile (96 Reactions)