VIASURE®E. coli EHEC, EPEC & EIEC Real Time PCR Detection Kit

The VIASURE E. coli EHEC + EPEC + EIEC Real Time PCR Detection Kit enables the simultaneous identification and differentiation of key diarrheagenic Escherichia coli pathotypes in stool samples, including:

• Enterohemorrhagic E. coli (EHEC)
• Enteropathogenic E. coli (EPEC)
• Enteroinvasive E. coli (EIEC)
• Shigella spp.

This ready-to-use Real-Time PCR assay includes lyophilized enzyme/primer-probe mix, internal control, and positive/negative controls. It supports rapid detection of infection, allowing for improved surveillance, targeted treatment, and public health response during gastrointestinal disease outbreaks.

Key Features & Benefits

• ✅ Multiplex Detection – Differentiates EHEC, EPEC, and EIEC/Shigella in one reaction
• ✅ Stabilized Reagents – Lyophilized 8-well strips or 96-well plates reduce pipetting and preparation errors
• ✅ Internal & External Controls – Built-in quality assurance to detect PCR inhibition or contamination
• ✅ Room Temperature Stability – Ideal for field use and simplified shipping logistics
• ✅ GLP/GMP Compatible – Traceable results with positive/negative controls included
• ✅ Flexible Formats – Compatible with most standard real-time PCR thermocyclers
• ✅ High Sensitivity & Specificity – Accurate results for clinical research and epidemiological studies

Applications

• Detection of diarrheagenic E. coli and Shigella in symptomatic patients
• Outbreak surveillance and response in healthcare or public health settings
• Foodborne illness monitoring
• Molecular epidemiology studies of E. coli pathotypes
• Clinical and translational research into enteric pathogens

Background Summary

Pathogenic E. coli are a major cause of diarrhoeal illness globally.

• EHEC/STEC produce Shiga toxins and cause diseases ranging from mild diarrhea to hemorrhagic colitis and hemolytic uremic syndrome (HUS).
• EPEC, although lacking Shiga toxins, causes attaching and effacing lesions in the small intestine, contributing to infantile diarrhea in developing regions.
• EIEC, closely related to Shigella spp., causes inflammatory colitis, and can present with fever, cramps, and bloody diarrhea.
• Transmission is mainly fecal-oral, often via contaminated food, water, or person-to-person contact.

Timely detection is essential to support outbreak control, targeted therapy, and prevent transmission.

Kit Contents

Ordering Information

The VIASURE E. coli EHEC + EPEC + EIEC Real Time PCR Detection Kit offers an accurate, rapid, and reliable molecular solution for identifying critical E. coli pathotypes and Shigella in clinical stool specimens. With its flexible format options, internal quality controls, and high-performance design, it supports efficient screening and monitoring of diarrheal disease-causing agents in both clinical and public health laboratories.