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Thermo Scientific™ Mass Spectrometry Grade Proteases are high-quality sequence-specific enzymes designed for efficient, reproducible protein digestion prior to LC-MS/MS protein identification, characterization, and quantification. These proteases cleave proteins into peptides suitable for mass spectrometry workflows, helping improve sequence coverage, peptide mapping, and analytical confidence.
The product range includes Trypsin, Lys-C, Trypsin/Lys-C Protease Mix, Asp-N, Glu-C, and Chymotrypsin, allowing researchers to select the most appropriate enzyme or digestion strategy for specific proteins, sample types, and MS applications.
Key Features
| Feature | Details |
|---|---|
| MS Grade Quality | Designed for mass spectrometry-based protein analysis |
| Sequence-Specific Digestion | Enables controlled cleavage of proteins into peptides |
| High Selectivity | Trypsin has >95% C-terminal lysine/arginine specificity; Lys-C has >90% C-terminal lysine specificity |
| Reduced Missed Cleavages | Trypsin/Lys-C mix improves digestion completeness |
| High Purity | Trypsin has no detectable chymotrypsin activity |
| Enhanced Stability | Chemically modified trypsin reduces autolytic activity |
| Flexible Protease Options | Includes trypsin, Lys-C, Asp-N, Glu-C, chymotrypsin, and Trypsin/Lys-C mix |
| Compatible Workflows | Suitable for in-solution and in-gel digestion workflows |
| Convenient Formats | Supplied in lyophilized or liquid formats, depending on product |
Applications
Thermo Scientific™ Mass Spectrometry Grade Proteases are suitable for:
- Protein digestion for LC-MS/MS analysis
- Protein identification and characterization
- Peptide mapping
- Proteomics research
- Protein quantification workflows
- In-solution protein digestion
- In-gel protein digestion
- Phosphopeptide enrichment workflows
- Sequential digestion strategies
- Improved protein sequence coverage
- Generation of complementary peptide fragments
- ETD and MS3-compatible peptide workflows
Protease Selection Guide
| Protease | Cleavage Specificity | Typical Use |
|---|---|---|
| Trypsin | C-terminal side of lysine and arginine residues | Routine protein digestion for LC-MS/MS |
| Lys-C | C-terminal side of lysine residues | Larger peptide generation, denaturing conditions, sequential digestion with trypsin |
| Trypsin/Lys-C Mix | Combined lysine/arginine digestion with reduced missed cleavages | Improved digestion efficiency and reproducibility |
| Asp-N | N-terminal side of aspartate and cysteic acid residues | Alternative digestion for complementary sequence coverage |
| Glu-C | C-terminal side of glutamic acid residues; may also cleave aspartic acid in phosphate buffers | Peptide mapping and complementary digestion |
| Chymotrypsin | C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine | Alternative digestion for improved sequence coverage |
Product Specifications
| Specification | Details |
|---|---|
| Product Name | Thermo Scientific™ Mass Spectrometry Grade Proteases |
| Product Line | Pierce™ |
| Final Product Type | Enzyme |
| Grade | MS |
| Workflow Step | Protein digestion |
| Detection Method | Mass spectrometry |
| For Use With | Mass spectrometer |
| Starting Material | Protein samples, cell lysate |
| Form | Lyophilized powder, with selected solution formats depending on product |
| Shipping Condition | Shipped with ice |
| Storage | -20 °C in a non-frost-free freezer |
| Unit Size | Each |
| Research Use | For Research Use Only; not for diagnostic procedures |
Ordering Information
| Catalog Number | Quantity | Product Type |
|---|---|---|
| 90057 | 5 x 20 µg | Trypsin Protease |
| 90053 | 2 µg | Asp-N Endoproteinase |
| 90056 | 4 x 25 µg | Chymotrypsin Endoproteinase |
| 90054 | 5 x 10 µg | Glu-C Endoproteinase |
| 90305 | 1 x 100 µg | Trypsin Protease |
| 90059 | 1 mg | Trypsin Protease |
| A40007 | 20 µg | Trypsin/Lys-C Protease Mix |
| A41007 | 5 x 20 µg | Trypsin/Lys-C Protease Mix |
| A40009 | 100 µg | Trypsin/Lys-C Protease Mix |
| 90051 | 1 x 20 µg | Lys-C Endoproteinase |
| 90307 | 100 µg | Lys-C Protease |
| 90058 | 5 x 100 µg | Trypsin Protease |
Protease Performance Overview
| Protease | Key Performance Details |
|---|---|
| Trypsin Protease | Porcine pancreatic MS-grade serine protease; TPCK-treated to remove chymotryptic activity; chemically modified by methylation for improved stability and reduced autolysis |
| Lys-C Protease | MS-grade serine protease from Lysobacter enzymogenes; cleaves lysine residues and remains active under highly denaturing conditions |
| Trypsin/Lys-C Protease Mix | Optimized lyophilized mix for improved protein digestion efficiency and fewer missed cleavages |
| Asp-N Protease | MS-grade zinc metalloproteinase; cleaves primarily at the amino side of aspartate and cysteic acid |
| Glu-C Protease | MS-grade V-8 serine protease from Staphylococcus aureus; cleaves glutamic acid residues under selected buffer conditions |
| Chymotrypsin Protease | MS-grade bovine pancreatic endoproteinase; cleaves aromatic and hydrophobic residues and is TLCK-treated to reduce tryptic activity |
Storage & Handling
Upon receipt, store at -20 °C in a non-frost-free freezer. Products are shipped with ice. Reconstitute and use according to the manufacturer’s recommended digestion protocol. Avoid repeated freeze-thaw cycles and use appropriate proteomics-grade reagents to minimize contamination and maximize digestion reproducibility.
Research Use Statement
For Research Use Only (RUO). Not for use in diagnostic procedures.
Product Sheet: Mass Spectrometry-Grade Endoproteinases
User Guide: Pierce Trypsin Protease, MS Grade
User Guide: Pierce Trypsin/Lys-C Protease Mix, MS Grade
Thermo Scientific™ Mass Spectrometry Grade Proteases are high-quality sequence-specific enzymes designed for efficient, reproducible protein digestion prior to LC-MS/MS protein identification, characterization, and quantification. These proteases cleave proteins into peptides suitable for mass spectrometry workflows, helping improve sequence coverage, peptide mapping, and analytical confidence.
The product range includes Trypsin, Lys-C, Trypsin/Lys-C Protease Mix, Asp-N, Glu-C, and Chymotrypsin, allowing researchers to select the most appropriate enzyme or digestion strategy for specific proteins, sample types, and MS applications.
Key Features
| Feature | Details |
|---|---|
| MS Grade Quality | Designed for mass spectrometry-based protein analysis |
| Sequence-Specific Digestion | Enables controlled cleavage of proteins into peptides |
| High Selectivity | Trypsin has >95% C-terminal lysine/arginine specificity; Lys-C has >90% C-terminal lysine specificity |
| Reduced Missed Cleavages | Trypsin/Lys-C mix improves digestion completeness |
| High Purity | Trypsin has no detectable chymotrypsin activity |
| Enhanced Stability | Chemically modified trypsin reduces autolytic activity |
| Flexible Protease Options | Includes trypsin, Lys-C, Asp-N, Glu-C, chymotrypsin, and Trypsin/Lys-C mix |
| Compatible Workflows | Suitable for in-solution and in-gel digestion workflows |
| Convenient Formats | Supplied in lyophilized or liquid formats, depending on product |
Applications
Thermo Scientific™ Mass Spectrometry Grade Proteases are suitable for:
- Protein digestion for LC-MS/MS analysis
- Protein identification and characterization
- Peptide mapping
- Proteomics research
- Protein quantification workflows
- In-solution protein digestion
- In-gel protein digestion
- Phosphopeptide enrichment workflows
- Sequential digestion strategies
- Improved protein sequence coverage
- Generation of complementary peptide fragments
- ETD and MS3-compatible peptide workflows
Protease Selection Guide
| Protease | Cleavage Specificity | Typical Use |
|---|---|---|
| Trypsin | C-terminal side of lysine and arginine residues | Routine protein digestion for LC-MS/MS |
| Lys-C | C-terminal side of lysine residues | Larger peptide generation, denaturing conditions, sequential digestion with trypsin |
| Trypsin/Lys-C Mix | Combined lysine/arginine digestion with reduced missed cleavages | Improved digestion efficiency and reproducibility |
| Asp-N | N-terminal side of aspartate and cysteic acid residues | Alternative digestion for complementary sequence coverage |
| Glu-C | C-terminal side of glutamic acid residues; may also cleave aspartic acid in phosphate buffers | Peptide mapping and complementary digestion |
| Chymotrypsin | C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine | Alternative digestion for improved sequence coverage |
Product Specifications
| Specification | Details |
|---|---|
| Product Name | Thermo Scientific™ Mass Spectrometry Grade Proteases |
| Product Line | Pierce™ |
| Final Product Type | Enzyme |
| Grade | MS |
| Workflow Step | Protein digestion |
| Detection Method | Mass spectrometry |
| For Use With | Mass spectrometer |
| Starting Material | Protein samples, cell lysate |
| Form | Lyophilized powder, with selected solution formats depending on product |
| Shipping Condition | Shipped with ice |
| Storage | -20 °C in a non-frost-free freezer |
| Unit Size | Each |
| Research Use | For Research Use Only; not for diagnostic procedures |
Ordering Information
| Catalog Number | Quantity | Product Type |
|---|---|---|
| 90057 | 5 x 20 µg | Trypsin Protease |
| 90053 | 2 µg | Asp-N Endoproteinase |
| 90056 | 4 x 25 µg | Chymotrypsin Endoproteinase |
| 90054 | 5 x 10 µg | Glu-C Endoproteinase |
| 90305 | 1 x 100 µg | Trypsin Protease |
| 90059 | 1 mg | Trypsin Protease |
| A40007 | 20 µg | Trypsin/Lys-C Protease Mix |
| A41007 | 5 x 20 µg | Trypsin/Lys-C Protease Mix |
| A40009 | 100 µg | Trypsin/Lys-C Protease Mix |
| 90051 | 1 x 20 µg | Lys-C Endoproteinase |
| 90307 | 100 µg | Lys-C Protease |
| 90058 | 5 x 100 µg | Trypsin Protease |
Protease Performance Overview
| Protease | Key Performance Details |
|---|---|
| Trypsin Protease | Porcine pancreatic MS-grade serine protease; TPCK-treated to remove chymotryptic activity; chemically modified by methylation for improved stability and reduced autolysis |
| Lys-C Protease | MS-grade serine protease from Lysobacter enzymogenes; cleaves lysine residues and remains active under highly denaturing conditions |
| Trypsin/Lys-C Protease Mix | Optimized lyophilized mix for improved protein digestion efficiency and fewer missed cleavages |
| Asp-N Protease | MS-grade zinc metalloproteinase; cleaves primarily at the amino side of aspartate and cysteic acid |
| Glu-C Protease | MS-grade V-8 serine protease from Staphylococcus aureus; cleaves glutamic acid residues under selected buffer conditions |
| Chymotrypsin Protease | MS-grade bovine pancreatic endoproteinase; cleaves aromatic and hydrophobic residues and is TLCK-treated to reduce tryptic activity |
Storage & Handling
Upon receipt, store at -20 °C in a non-frost-free freezer. Products are shipped with ice. Reconstitute and use according to the manufacturer’s recommended digestion protocol. Avoid repeated freeze-thaw cycles and use appropriate proteomics-grade reagents to minimize contamination and maximize digestion reproducibility.
Research Use Statement
For Research Use Only (RUO). Not for use in diagnostic procedures.
Product Sheet: Mass Spectrometry-Grade Endoproteinases
User Guide: Pierce Trypsin Protease, MS Grade
User Guide: Pierce Trypsin/Lys-C Protease Mix, MS Grade
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Specifications
| Protease | Trypsin Protease, 5 x 20 µg, Trypsin Protease, 1 mg, Trypsin Protease, 5 x 100 µg, Trypsin Protease, 1 x 100 µg, Asp-N Endoproteinase, 2 µg, Chymotrypsin Endoproteinase, 4 x 25 µg, Glu-C Endoproteinase, 5 x 10 µg, Trypsin/Lys-C Protease Mix, 20 µg, Trypsin/Lys-C Protease Mix, 5 x 20 µg, Trypsin/Lys-C Protease Mix, 100 µg, Lys-C Endoproteinase, 1 x 20 µg, Lys-C Protease, 100 µg |