Thermo Scientific™ Pierce™ Protease Inhibitor XL Capsules, EDTA-free

Thermo Scientific™ Pierce™ Protease Inhibitor XL Capsules, EDTA-free, are designed for efficient and reliable protection of proteins during large-volume cell lysis and protein extraction procedures. Each capsule contains a broad-spectrum formulation of protease inhibitors and is sufficient for preparing up to 500 mL of solution, making it ideal for high-throughput and scale-up applications.

These capsules provide a convenient, ready-to-use alternative to traditional inhibitors such as PMSF, offering enhanced protection across multiple protease classes while maintaining compatibility with downstream protein analysis workflows.

Key Features

Broad-Spectrum Protease Inhibition

• Inhibits serine, cysteine, aspartic proteases, and aminopeptidases
• Provides stronger and more comprehensive protection than PMSF alone
• Preserves protein integrity during extraction and processing

Large-Volume Format

• One capsule sufficient for 500 mL of buffer or lysate
• Ideal for bulk sample preparation and high-throughput workflows
• Reduces preparation time and reagent handling

EDTA-Free Formulation

• Suitable for applications involving metalloproteins and metal-dependent enzymes
• Prevents interference with metal-sensitive assays

Convenient and Fast Dissolution

• Capsule format allows easy handling and dosing
• Dissolves completely within approximately 3 minutes
• Produces a clear solution ready for immediate use

Compatible with Downstream Applications

• Compatible with Pierce protein assays
• Suitable for detergent-based lysis buffers
• Can be combined with phosphatase inhibitors if required

Applications

• Large-volume protein extraction from tissues and cultured cells
• High-throughput proteomics workflows
• Cell lysis and sample preparation
• Western blotting and immunoassays
• Protein purification and enzyme studies

Mechanism of Action

During cell lysis, endogenous proteases are released and can degrade target proteins. Pierce Protease Inhibitor XL Capsules contain a combination of inhibitors that act on different protease classes:

• AEBSF – inhibits serine proteases
• Bestatin – inhibits aminopeptidases
• E-64 – inhibits cysteine proteases
• Pepstatin A – inhibits aspartic proteases

This combination ensures broad-spectrum inhibition and effective protection of proteins during large-scale extraction processes.

Technical Specifications

Usage Instructions

• Twist open one capsule
• Add contents to 500 mL of buffer or lysate
• Mix until fully dissolved (approximately 3 minutes)
• Use immediately in protein extraction workflow

Product Information

Storage and Stability

• Store at 2°C to 8°C
• Maintain in tightly sealed container
• Protect from moisture

Related Products

• Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
• Halt Phosphatase Inhibitor Cocktail, EDTA-free (100X)
• T-PER Tissue Protein Extraction Reagent

Thermo Scientific™ Pierce™ Protease Inhibitor XL Capsules, EDTA-free, offer a highly efficient and scalable solution for protecting proteins during large-volume extraction workflows. With broad-spectrum inhibition, convenient capsule format, and compatibility with downstream assays, they are an essential reagent for laboratories performing high-throughput proteomics and protein research.