Thermo Scientific™ HpyF3I (DdeI) (10 U/μL)

The Thermo Scientific™ HpyF3I (DdeI) Restriction Enzyme is a high-quality molecular biology tool used for cleaving DNA at specific recognition sites. This enzyme recognizes the sequence C^TNAG (where ^ indicates the cutting site) and performs best at a temperature of 37°C when used with the Tango buffer. The enzyme is part of Thermo Fisher Scientific's extensive collection of conventional restriction endonucleases, optimized for use in a variety of molecular biology applications.

Key Features & Benefits:

• High Quality: Thermo Scientific restriction enzymes are manufactured under stringent quality control to ensure consistent, reliable performance in your experiments.
• Convenient Buffers: Includes the universal Tango buffer for use in double digestion reactions, as well as the Five Buffer System for various enzyme requirements.
• Premixed BSA: All reaction buffers are premixed with Bovine Serum Albumin (BSA), which helps stabilize the enzyme and bind contaminants that may be present in DNA preparations.
• Optimal Performance: Designed for use at an optimal reaction temperature of 37°C, ensuring the highest enzyme activity.
• Methylation Sensitivity: The enzyme is not sensitive to Dam, Dcm, or CpG methylation, ensuring reliability for a wide range of applications.

Applications:

• Molecular Cloning: Essential for cutting DNA at precise locations, facilitating gene insertion into vectors.
• Restriction Site Mapping: Helps identify and map specific DNA sequences.
• Genotyping: Useful for DNA profiling and gene sequence analysis.
• Southern Blotting: Useful for analyzing DNA fragments after digestion and transfer.
• RFLP (Restriction Fragment Length Polymorphism): Used to analyze genetic variation based on restriction enzyme digestion patterns.
• SNP Analysis: Facilitates the study of single nucleotide polymorphisms by cutting DNA at known sites.

Specifications:

• Concentration: 10 U/μL
• Methylation Sensitivity: Not sensitive to Dam, Dcm, or CpG methylation.
• Enzyme: HpyF3I (DdeI)
• Buffer Compatibility: Compatible with 10X Buffer Tango.
• Sensitive to Heat Inactivation: Yes, heat inactivation is required.
• Optimal Reaction Temperature: 37°C
• Type IIS RE: No, it is not a Type IIS restriction enzyme.
• Quantity: 2,500 units or 500 units (depending on the catalog number).
• Product Type: Restriction enzyme for DNA digestion.

Catalog Numbers and Available Quantities:

Storage and Handling:

• Storage: Store at -20°C for long-term storage.
• Heat Inactivation: Enzyme activity can be heat-inactivated as per recommended protocols.

Important Notes:

• For methylation sensitivity, refer to the specific product specifications.
• This enzyme is for research use only and is not for use in diagnostic procedures.