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Hin1II (also known as NlaIII) is a high-quality restriction endonuclease from Thermo Scientific™ that recognizes the DNA sequence 5′-CATG^-3′ / 3′-^GTAC-5′ and cleaves at the indicated site. It delivers optimal performance at 37°C in Buffer G and is an essential tool in molecular biology for applications such as cloning, genotyping, RFLP, and SNP analysis.
Hin1II is part of Thermo Scientific’s comprehensive range of conventional restriction enzymes, which are validated under stringent quality control measures and optimized to work with the Five Buffer System, including the universal Tango buffer for double digestions. This enzyme is also available in FastDigest™ format for rapid DNA digestion protocols.
Key Features
- Recognition Site: 5′-CATG^-3′
- Cutting Efficiency: 100% activity in recommended buffer (Buffer G)
- Concentration: 5 U/μL, supplied as 300 units per vial (Catalog No. ER1831)
- Storage Buffer: Supplied in Thermo Scientific’s color-coded Five Buffer System with pre-mixed BSA
- Superior QC Standards: Manufactured with high stringency and consistency
- Isoschizomers: CviAII, FaeI, FatI, Hsp92II, NlaIII
- Heat Inactivation: Sensitive to heat inactivation; refer to protocol for conditions
Applications
- Molecular Cloning – Precise insertion of DNA fragments
- Restriction Mapping – Analysis of plasmid/vector integrity
- Genotyping & SNP Detection – Targeted digestion for polymorphism studies
- RFLP & Southern Blotting – Fragmentation of genomic DNA for hybridization assays
Specifications Table
| Attribute | Details |
|---|---|
| Product Name | Hin1II (NlaIII) Restriction Enzyme |
| Catalog Number | ER1831 |
| Recognition Sequence | 5′-CATG^–3′ / 3′–^GTAC–5′ |
| Quantity | 300 units |
| Concentration | 5 U/μL |
| Optimal Temperature | 37°C |
| Compatible Buffer | 10X Buffer G (Five Buffer System) |
| Heat Inactivation | Yes |
| Methylation Sensitivity | Not sensitive to Dam, Dcm, or CpG methylation |
| Format | Liquid enzyme with premixed BSA |
| Type IIS | No |
| For Use In | Research only (not for diagnostic use) |
Ordering Information
| Catalog Number | Description | Pack Size |
|---|---|---|
| ER1831 | Hin1II (NlaIII), 5 U/μL | 300 Units |
The Thermo Scientific™ Hin1II (NlaIII) restriction enzyme is a trusted, high-performance tool for precise DNA cleavage at CATG sites. Backed by rigorous quality control and compatibility with Thermo’s Five Buffer System, this enzyme is ideal for standard and advanced molecular biology workflows, including cloning, mapping, and genotyping.
User Guide: Hin1II (NlaIII), 5 U/uL, 300U
Hin1II (also known as NlaIII) is a high-quality restriction endonuclease from Thermo Scientific™ that recognizes the DNA sequence 5′-CATG^-3′ / 3′-^GTAC-5′ and cleaves at the indicated site. It delivers optimal performance at 37°C in Buffer G and is an essential tool in molecular biology for applications such as cloning, genotyping, RFLP, and SNP analysis.
Hin1II is part of Thermo Scientific’s comprehensive range of conventional restriction enzymes, which are validated under stringent quality control measures and optimized to work with the Five Buffer System, including the universal Tango buffer for double digestions. This enzyme is also available in FastDigest™ format for rapid DNA digestion protocols.
Key Features
- Recognition Site: 5′-CATG^-3′
- Cutting Efficiency: 100% activity in recommended buffer (Buffer G)
- Concentration: 5 U/μL, supplied as 300 units per vial (Catalog No. ER1831)
- Storage Buffer: Supplied in Thermo Scientific’s color-coded Five Buffer System with pre-mixed BSA
- Superior QC Standards: Manufactured with high stringency and consistency
- Isoschizomers: CviAII, FaeI, FatI, Hsp92II, NlaIII
- Heat Inactivation: Sensitive to heat inactivation; refer to protocol for conditions
Applications
- Molecular Cloning – Precise insertion of DNA fragments
- Restriction Mapping – Analysis of plasmid/vector integrity
- Genotyping & SNP Detection – Targeted digestion for polymorphism studies
- RFLP & Southern Blotting – Fragmentation of genomic DNA for hybridization assays
Specifications Table
| Attribute | Details |
|---|---|
| Product Name | Hin1II (NlaIII) Restriction Enzyme |
| Catalog Number | ER1831 |
| Recognition Sequence | 5′-CATG^–3′ / 3′–^GTAC–5′ |
| Quantity | 300 units |
| Concentration | 5 U/μL |
| Optimal Temperature | 37°C |
| Compatible Buffer | 10X Buffer G (Five Buffer System) |
| Heat Inactivation | Yes |
| Methylation Sensitivity | Not sensitive to Dam, Dcm, or CpG methylation |
| Format | Liquid enzyme with premixed BSA |
| Type IIS | No |
| For Use In | Research only (not for diagnostic use) |
Ordering Information
| Catalog Number | Description | Pack Size |
|---|---|---|
| ER1831 | Hin1II (NlaIII), 5 U/μL | 300 Units |
The Thermo Scientific™ Hin1II (NlaIII) restriction enzyme is a trusted, high-performance tool for precise DNA cleavage at CATG sites. Backed by rigorous quality control and compatibility with Thermo’s Five Buffer System, this enzyme is ideal for standard and advanced molecular biology workflows, including cloning, mapping, and genotyping.
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