Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin is a high-quality, water-soluble, amine-reactive biotinylation reagent designed for efficient labeling of proteins, antibodies, and other amine-containing molecules in aqueous buffer systems. This reagent incorporates a medium-length spacer arm (22.4 Å) and is ideal for applications requiring robust, non-cleavable labeling without disrupting protein structure or function.

Key Features and Benefits

• Selective Protein Labeling: Targets primary amines (lysine residues and N-termini) for biotinylation, enabling downstream detection or immobilization using avidin, streptavidin, or NeutrAvidin™ reagents.
• Water-Soluble and Cell-Impermeant: The sulfo-NHS group increases water solubility and prevents membrane penetration, making it ideal for cell surface protein labeling without entering the cytoplasm.
• Medium-Length Spacer Arm (22.4 Å): Minimizes steric hindrance during avidin–biotin binding interactions, ensuring high binding efficiency without excessive mass addition.
• Irreversible Covalent Bonding: Forms stable amide bonds that cannot be cleaved, ensuring permanent biotin conjugation.
• Ready-to-Use: Easily dissolved in aqueous buffers without the need for organic solvents such as DMSO or DMF, unlike traditional NHS-ester biotin reagents.

Applications

• Antibody Biotinylation: For detection, purification, or immobilization in ELISA, Western blot, immunoprecipitation, and flow cytometry.
• Cell Surface Protein Labeling: Used in live-cell experiments where membrane-impermeant reagents are essential.
• Affinity Purification and Pull-Down Assays: Ideal for immobilizing biotinylated targets on streptavidin-coated beads or plates.
• Protein-Protein Interaction Studies: Enables site-directed biotinylation for biosensor analysis (e.g., Biacore, Octet).
• Enzyme and Ligand Labeling: Useful in developing biotin-tagged probes and reagents for activity-based profiling.

Technical Specifications

Kit Options Available

• EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit – All-in-one kit including buffers and controls
• EZ-Link™ Micro Sulfo-NHS-LC-Biotinylation Kit – Scaled for low-volume or high-value samples

EZ-Link™ Sulfo-NHS-LC-Biotin offers a reliable, versatile, and high-performance solution for researchers needing effective biotin labeling of proteins and antibodies. Its balance of water solubility, cell impermeability, and optimal spacer length makes it particularly useful for surface labeling and quantitative detection applications in proteomics, immunology, and cell biology.