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The Thermo Scientific™ Pierce™ C18 Spin Tips and Spin Columns are high-efficiency, ready-to-use tools for the desalting, cleanup, and enrichment of peptides prior to mass spectrometry (MS) analysis. They employ a porous reverse-phase C18 resin or monolithic sorbent, enabling the rapid and reproducible removal of interfering substances such as salts, detergents, and other MS-incompatible compounds. Designed for flexibility, the format supports a wide range of peptide sample volumes and concentrations, ensuring excellent peptide recovery and improved spectral quality for MALDI-MS and ESI-MS.
Key Features & Benefits
-
High Recovery & Sensitivity
Retains and elutes peptides efficiently—even from sub-picomole quantities of digested protein. -
Rapid & Convenient Sample Prep
Complete cleanup and elution in under five minutes using a simple 5-step spin protocol. -
MS-Compatible Output
Peptides are eluted in solvents suitable for LC-MS/MS or MALDI-MS, free from salts or detergents. -
Flexible Volumes & Formats
Available in both spin tip and pipette tip formats for manual or automated workflows. -
Centrifuge-Compatible Design
Spin tips include adaptors for easy microcentrifuge processing without special equipment. -
Low Dead Volume & Minimal Loss
Engineered to prevent sample loss during loading or elution, ensuring maximum peptide recovery.
Applications
- Peptide desalting and sample prep for MALDI-MS or LC-MS/MS
- Cleanup of peptide mixtures post-enzymatic digestion
- Concentration of dilute peptide solutions
- Removal of buffers, salts, and surfactants before MS analysis
- High-throughput proteomics workflows and biomarker discovery
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Spin Tip / Pipette Tip / Spin Column |
| Sorbent | C18 Reverse-Phase Resin / Monolithic C18 |
| Binding Capacity | Up to 10 μg peptides per 20 μL sample |
| Volume Range | 10 – 100 μL (Spin Tips) |
| Format | Spin Tips (84850), 10 μL Tips (87782), 100 μL Tips (87784) |
| Sample Type | Protease-digested proteins, peptides |
| Compatible Equipment | Microcentrifuge, Mass Spectrometer |
| Workflow Step | Sample Clean-up, Peptide Desalting |
| Detection Method | Mass Spectrometry (MALDI-MS, ESI-MS) |
| Storage Conditions | Room temperature |
The Pierce™ C18 Spin Tips and Spin Columns offer an efficient and reproducible method to clean up peptide samples before mass spectrometry. Their user-friendly design, high recovery rate, and compatibility with common MS platforms make them a staple in proteomics, biomarker validation, and protein characterization workflows.
Quantitative Profiling of DNA Damage Response Proteins Using iTRAQ Labeling and the LTQ Orbitrap XL
User Guide: Pierce C18 Spin Tips
User Guide: Pierce C18 Tips
The Thermo Scientific™ Pierce™ C18 Spin Tips and Spin Columns are high-efficiency, ready-to-use tools for the desalting, cleanup, and enrichment of peptides prior to mass spectrometry (MS) analysis. They employ a porous reverse-phase C18 resin or monolithic sorbent, enabling the rapid and reproducible removal of interfering substances such as salts, detergents, and other MS-incompatible compounds. Designed for flexibility, the format supports a wide range of peptide sample volumes and concentrations, ensuring excellent peptide recovery and improved spectral quality for MALDI-MS and ESI-MS.
Key Features & Benefits
-
High Recovery & Sensitivity
Retains and elutes peptides efficiently—even from sub-picomole quantities of digested protein. -
Rapid & Convenient Sample Prep
Complete cleanup and elution in under five minutes using a simple 5-step spin protocol. -
MS-Compatible Output
Peptides are eluted in solvents suitable for LC-MS/MS or MALDI-MS, free from salts or detergents. -
Flexible Volumes & Formats
Available in both spin tip and pipette tip formats for manual or automated workflows. -
Centrifuge-Compatible Design
Spin tips include adaptors for easy microcentrifuge processing without special equipment. -
Low Dead Volume & Minimal Loss
Engineered to prevent sample loss during loading or elution, ensuring maximum peptide recovery.
Applications
- Peptide desalting and sample prep for MALDI-MS or LC-MS/MS
- Cleanup of peptide mixtures post-enzymatic digestion
- Concentration of dilute peptide solutions
- Removal of buffers, salts, and surfactants before MS analysis
- High-throughput proteomics workflows and biomarker discovery
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Spin Tip / Pipette Tip / Spin Column |
| Sorbent | C18 Reverse-Phase Resin / Monolithic C18 |
| Binding Capacity | Up to 10 μg peptides per 20 μL sample |
| Volume Range | 10 – 100 μL (Spin Tips) |
| Format | Spin Tips (84850), 10 μL Tips (87782), 100 μL Tips (87784) |
| Sample Type | Protease-digested proteins, peptides |
| Compatible Equipment | Microcentrifuge, Mass Spectrometer |
| Workflow Step | Sample Clean-up, Peptide Desalting |
| Detection Method | Mass Spectrometry (MALDI-MS, ESI-MS) |
| Storage Conditions | Room temperature |
The Pierce™ C18 Spin Tips and Spin Columns offer an efficient and reproducible method to clean up peptide samples before mass spectrometry. Their user-friendly design, high recovery rate, and compatibility with common MS platforms make them a staple in proteomics, biomarker validation, and protein characterization workflows.
Quantitative Profiling of DNA Damage Response Proteins Using iTRAQ Labeling and the LTQ Orbitrap XL
User Guide: Pierce C18 Spin Tips
User Guide: Pierce C18 Tips
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Specifications
| Pack Size | 96 Tips package, 96 x 10 μL Tips, 96 x 100 μL Tips |