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Thermo Scientific™ Tween 20 Blocking Buffer, 1% in PBS (10X)
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Concentrated PBS-T Blocking and Wash Buffer for Western Blotting and ELISA
Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | Ambient | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Tween 20 Blocking Buffer, 1% in PBS (10X) is a high-quality concentrated buffer solution formulated for effective washing and blocking in antibody-based immunoassays. This PBS-based formulation contains 1% Tween 20, a gentle nonionic surfactant that reduces nonspecific antibody binding and improves assay clarity.
Designed for routine use in Western blotting, ELISA, and other immunodetection protocols, this blocking buffer helps minimize background staining by preventing unwanted protein interactions and ensuring efficient removal of unbound immunological reagents between incubation steps.
Supplied as a viscous liquid 10X concentrate, it offers convenience, reproducibility, and reduced preparation time in busy research laboratories.
Key Features and Benefits
Prevents Nonspecific Antibody Binding
Tween 20 blocks nonspecific binding sites, improving signal-to-noise ratio and reducing background in immunoassays.
Optimized for Wash and Blocking Steps
Ideal for washes between immunoreactions and for preparing incubation solutions of labeled antibodies or immunoreagents.
Efficient Removal of Unbound Reagents
Helps remove excess antibodies and proteins from membranes and microplates, enhancing detection specificity.
Convenient Concentrated Stock Solution
Supplied as a 10X concentrate, allowing rapid dilution to working strength and consistent buffer preparation.
Cold Storage for Stability
Recommended storage at 2°C to 8°C ensures optimal performance over time.
Applications
Thermo Scientific™ Tween 20 Blocking Buffer is widely used for:
- Western blot membrane washing and blocking
- ELISA plate washing steps
- Immunoassay background reduction
- Removal of unbound immunological reagents
- Antibody incubation solution preparation
- General immunodetection workflows
Product Specifications
| Parameter | Description |
|---|---|
| Product Name | Tween 20 Blocking Buffer |
| Composition | 1% Tween 20 in PBS |
| Concentration | 10X Stock Solution |
| Physical Form | Viscous liquid |
| Chemical Type | Blocking Buffer |
| Recommended Storage | 2°C to 8°C |
| Primary Use | Western blot, ELISA, immunoassays |
Ordering Information
| Catalog Number | Pack Size | Alternate Name |
|---|---|---|
| J61544.AP | 500 mL | J61544-AP |
| J61544.K2 | 1 L | J61544-K2 |
| J61544.K3 | 2 L | J61544-K3 |
Thermo Scientific™ Tween 20 Blocking Buffer, 1% in PBS (10X), is a reliable and effective solution for reducing nonspecific antibody binding and improving washing efficiency in Western blotting, ELISA, and other immunoassays. With its optimized detergent formulation, convenient concentrated format, and cold storage stability, it is an essential reagent for laboratories seeking reproducible, low-background immunodetection results.
- Pack Size: 1L 500 mL 2L