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Thermo Scientific™ Pierce™ Protein L Agarose
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Specifications:
| Application | Protein Purification | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Pierce Protein L Agarose consists of recombinant Protein L covalently immobilized onto high-quality crosslinked 6% beaded agarose (CL-6B). Protein L binds to immunoglobulins through their kappa light chains, rather than the Fc region. This enables purification across multiple antibody classes—including IgG, IgM, IgA, IgE, and IgD—provided they contain the compatible kappa light chain types.
It is especially useful for purifying Fab fragments, scFv antibodies, and monoclonal antibodies produced in culture media supplemented with bovine serum, since Protein L does not bind bovine immunoglobulins. Additionally, it supports immunoprecipitation and co-immunoprecipitation assays without interfering with the antigen-binding region.
Key Features & Benefits
- Kappa Light Chain Specificity: Binds to human and mouse antibodies containing compatible kappa light chains (e.g., human VkI, VkIII, VkIV; mouse VkI).
- High Purity and Recovery: Purifies 5–10 mg of IgG per mL of resin with low nonspecific binding.
- Wide Ig Class Compatibility: Supports purification of IgG, IgA, IgM, IgE, and IgD subclasses with kappa chains.
- Ideal for Fragment Purification: Binds scFv and Fab fragments, making it suitable for structural and functional studies.
- Low Background in Complex Media: Does not bind bovine IgG, enabling effective antibody capture from serum-containing media.
- Stability & Reusability: Resin remains stable across a wide pH range (2–14 short term) and is suitable for multiple purification cycles.
Technical Specifications
| Feature | Details |
|---|---|
| Binding Ligand | Recombinant Protein L |
| Binding Capacity | 5–10 mg human IgG/mL resin |
| Resin Support | Crosslinked 6% beaded agarose (CL-6B) |
| Particle Size | 45–165 μm |
| pH Stability | 2–14 (short-term); 3–13 (long-term) |
| Exclusion Limit | 10,000–4,000,000 Daltons |
| Format | Bottle (Liquid Suspension) |
| Quantity | 2 mL |
| Storage | 4°C to 8°C |
| Shipping Condition | Ambient temperature |
Applications
- Purification of mouse and human monoclonal antibodies with kappa chains
- Recovery of scFv and Fab fragments from expression systems
- Co-immunoprecipitation (Co-IP) and immunoprecipitation (IP) experiments
- Antibody purification from serum-containing hybridoma cultures
- Purification of Ig isotypes that do not bind to Protein A or G
Thermo Scientific™ Pierce™ Protein L Agarose is a versatile, high-performance affinity resin tailored for immunoglobulin purification where traditional Protein A or G resins fall short. Its unique binding specificity, combined with a robust agarose support matrix and efficient covalent immobilization chemistry, makes it an essential tool for modern antibody discovery, development, and proteomics workflows.
- Pack Size: 10 mL 2 mL