Thermo Scientific™ Pierce™ Protein A IgG Binding Buffer, pH 8.0; contains EDTA as a preservative

Thermo Scientific™ Pierce™ Protein A IgG Binding Buffer is a phosphate-based buffer solution, optimized for non-denaturing binding of IgG antibodies to Protein A affinity supports. Supplied as a 1X ready-to-use formulation, it contains glycerol and EDTA to protect proteins from oxidation and degradation during purification.

This buffer is specifically designed for use with Protein A agarose or resin-based purification systems, and is ideal for laboratory workflows involving antibody capture, affinity chromatography, and Fc-specific binding from serum, ascites, or hybridoma supernatants.

Key Features & Benefits

• Optimized for Protein A Affinity Binding
  Provides optimal pH and ionic strength for selective binding of IgG to Protein A.
• Stabilizing Additives
  Contains glycerol and EDTA to maintain antibody integrity and reduce protease activity.
• Ready-to-Use Format
  Pre-formulated and prefiltered; no dilution or preparation required.
• Consistent, High-Yield Results
  Ensures reproducible IgG capture without protein denaturation.
• Non-Toxic and Assay-Compatible
  Suitable for downstream applications such as ELISA, Western blotting, and mass spectrometry.

Technical Specifications

Applications

• IgG Purification
  Binding of monoclonal or polyclonal IgG to Protein A columns from serum or hybridoma culture.
• Affinity Chromatography Setup
  Used during the loading phase in antibody purification protocols.
• Protein Complex Capture
  Enables efficient capture of Fc-containing complexes or antibody-tagged targets.
• Proteomics & Immunology Workflows
  Suitable for antibody immobilization and immunoprecipitation.

Thermo Scientific™ Pierce™ Protein A IgG Binding Buffer is a trusted reagent for achieving high-yield, high-fidelity antibody capture in Protein A-based purification workflows. With a carefully optimized formulation that stabilizes antibodies and facilitates strong, specific binding, it is ideal for researchers conducting routine immunoglobulin purification, antibody-based assays, or downstream protein interaction studies.