Thermo Scientific™ Pierce™ PNPP Substrates

High-Performance PNPP Substrates for ELISA Applications 

Thermo Scientific™ Pierce PNPP Substrates are available in multiple formats, including powders, tablets, solutions, and complete kits. These substrates contain p-nitrophenyl phosphate (PNPP), a widely used colorimetric substrate for alkaline phosphatase-based ELISA applications. Upon reaction with alkaline phosphatase, PNPP generates a yellow water-soluble reaction product that absorbs light at 405 nm, allowing for precise and reliable detection.

Key Features & Benefits:

✅ One-Component Alkaline Phosphatase (AP) Substrate

• Simplifies ELISA workflows by eliminating the need for additional reagents.
• Stable and easy to handle.

✅ Reliable Absorbance at 405 nm

• Generates a clear, yellow color upon reaction with alkaline phosphatase.
• Ensures consistent and accurate colorimetric detection.

✅ Multiple Format Options for Flexibility

• Available as dry powder, single-use tablets, stable substrate solutions, and complete kits.
• Meets various experimental needs for ELISA applications.

✅ Safer to Use

• Contains a non-mercury, non-azide preservative for improved safety and environmental compatibility.

Available Formats & Catalog Numbers:

Product Overview: PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is an efficient substrate for detecting alkaline phosphatase in ELISA applications. The yellow reaction product formed upon enzymatic hydrolysis is highly soluble and provides reliable spectrophotometric readings at 405 nm. Thermo Scientific™ Pierce PNPP substrates are available in several convenient formats:

✅ PNPP Powder

• Dry crystalline form for custom preparation.
• Provides flexibility for different buffer conditions.

✅ PNPP Substrate Tablets

• 5 mg tablets with an inert filler for precise and reproducible substrate preparation.
• Eliminates the need for weighing, improving consistency.

✅ PNPP Substrate Kit

• Includes PNPP tablets and a Diethanolamine Buffer for the preparation of more than one liter of substrate.
• Also available separately: Diethanolamine Substrate Buffer (Cat. No. 34064) as a 5X concentrate.

✅ 1-Step™ PNPP Substrate Solution

• Ready-to-use, single-component substrate for fast and convenient ELISA workflows.
• Stable for up to 12 months at 2–8°C.
• Can be stopped with conventional stop solutions for endpoint readings.

Specifications:

Recommended Applications:

• ELISA-based protein quantification
• Immunoassays utilizing alkaline phosphatase detection
• High-throughput screening requiring colorimetric readout
• Enzyme activity assays in research and diagnostic applications

Thermo Scientific™ PNPP Substrates provide a versatile and reliable solution for alkaline phosphatase-based ELISA applications. Available in multiple convenient formats, these substrates support a wide range of laboratory workflows. Whether you require a ready-to-use solution or a complete kit for large-scale experiments, PNPP substrates deliver precise, reproducible results.

For assistance with product selection, bulk orders, or pricing details, please contact LabMart Limited. We’re here to support your research success!