Thermo Scientific™ Pierce™ Bradford Plus Protein Assay Kit

Catalog No :

CAS Number :

Brand :

Availability :

In Stock

Sufficient for 630 tube assays or 3160 microplate assays

Components
- Kit with BSA Standard (2 mg/mL, 10 × 1 mL) + 7,999.54 GH¢
- Kit with Pre-Diluted BSA Standards (7-point curve: 0.125 to 2 mg/mL) + 7,399.65 GH¢

This combination does not exist.

Specifications:

Application	Protein Assays & Analysis
Storage Temperature	2-8°C
Product Type	Assay Kits	Forms	Kit with Various components
Product Brand	Thermo Fisher Scientific™
Product Grade	Molecular Biology

Overview
Resource

The Thermo Scientific™ Pierce™ Bradford Plus Protein Assay Kit is a next-generation, colorimetric protein quantitation assay based on the Coomassie (Bradford) dye-binding method, optimized for improved linearity, reduced protein-to-protein variability, and compatibility with reducing agents.

This ready-to-use formulation enables rapid and reproducible detection of total protein concentration in a wide range of sample types, including those containing common interfering substances like DTT, TCEP, salts, buffers, and metal chelators.

Kits are available with either:

A standard BSA solution (2 mg/mL) (Cat. No. 23236), or
A Dilution-Free BSA Standard Set (Cat. No. A55866) containing seven pre-diluted BSA concentrations for immediate use in standard curve generation—ideal for high-throughput microplate assays using multichannel pipettes.

Key Features & Benefits

Improved Linearity & Reproducibility
Less protein-to-protein variation compared to traditional Bradford reagents.
Reducing Agent Compatibility
Compatible with DTT, TCEP, and other common reducing agents not tolerated by standard Bradford reagents.
Rapid, One-Step Protocol
No reagent mixing or incubation delays—add, mix, read.
Flexible Format
Suitable for cuvette- or microplate-based detection; supports low- and high-throughput workflows.
Wide Assay Range
Detects total protein between 1 to 1500 µg/mL—ideal for lysates, extracts, and purified protein samples.
Colorimetric Detection
Measure absorbance at 595 nm using any standard spectrophotometer or plate reader.
BSA Standards Included
Choose between a single-concentration or a pre-diluted 7-point BSA standard curve.

Technical Specifications

Property	Details
Assay Type	Bradford (Coomassie dye-based)
Detection Method	Colorimetric (595 nm)
Assay Range	1 – 1500 µg/mL
Sample Compatibility	Buffers, thiols, detergents, metal chelators
Protocol Format	Tube or 96-well microplate
Reagent Volume (23236)	950 mL
BSA Standard Concentration	2 mg/mL (23236); 0.125–2 mg/mL (A55866)
Kit Format (A55866)	8-tube strip with 7 standards + 1 blank
Sufficient For	630 tube assays or 3160 microplate assays
Storage	Reagent: 4°C; BSA Standards: Room temperature
Intended Use	For Research Use Only

Applications

Total Protein Quantification
Quantify lysates, serum, purified protein, and crude extracts for downstream analysis (e.g., SDS-PAGE, Western blot).
Standard Curve Generation
Use included BSA standards for reliable calibration in each run.
Assay Development & QC
Measure total protein for method development, normalization, and recovery validation.
Protein Normalization
Standardize protein concentration for enzyme assays, immunoassays, and bioanalytical workflows.

Kit Components

Pierce Bradford Plus Protein Assay Kit (Catalog No. 23236)

Pierce Bradford Plus Reagent, 950 mL
BSA Standard (2 mg/mL), 10 × 1 mL ampules

Pierce Bradford Plus Protein Assay Kit with Dilution-Free Standards (Catalog No. A55866)

Pierce Bradford Plus Reagent
Pre-diluted BSA Standards: 0.125, 0.25, 0.5, 0.75, 1.0, 1.5, 2.0 mg/mL
Multichannel-compatible 8-tube strip (includes buffer blank)

The Pierce™ Bradford Plus Protein Assay Kit provides a robust, sensitive, and highly reproducible method for quantifying total protein concentration in a wide range of research applications. With enhanced linearity, reducing agent compatibility, and optional pre-diluted BSA standards, this kit is the ideal solution for laboratories that demand reliable and rapid protein quantitation across low-to-moderate sample concentrations.