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Thermo Scientific™ Nalgene™ 5-Liter Shake Flask
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Specifications:
| Application | Cell culture, Bioprocessing |
| Storage Temperature | Room Temperature |
| Product Type | flasks |
| Product Brand | Thermo Fisher Scientific™, Nalgene |
| Product Grade | Cell Culture |
The Thermo Scientific™ Nalgene™ 5-Liter Shake Flasks are high-quality, single-use PETG flasks designed for large-scale suspension cell culture. These flasks are available in two bottom designs:
- Faceted-bottom flasks (Cat. No. 4115-5001) – Support the culture of a wide variety of cell types.
- Angled-bottom flasks (Cat. No. 4115-5000) – Specifically designed for scaling up sensitive cell types and transient transfection with the ExpiCHO Expression System.
Manufactured from break-resistant PETG, these sterile, non-pyrogenic, and non-cytotoxic shake flasks provide optimal gas exchange, secure handling, and quick access via peel-open packaging, making them ideal for biotechnology, pharmaceutical, and research laboratories.
Key Features & Benefits
1. Large-Scale Suspension Cell Culture for High-Yield Production
- 5 L capacity enables efficient expansion of cell cultures.
- Wide liquid surface area ensures maximum gas exchange for optimal cell growth.
- Compatible with bioreactor systems for large-scale production.
2. Two Bottom Designs for Versatile Applications
- Faceted-bottom flasks (4115-5001): Suitable for a wide range of cell types.
- Angled-bottom flasks (4115-5000): Optimized for ExpiCHO transient transfection and sensitive cell lines.
3. High-Quality PETG Construction for Maximum Durability
- Crystal-clear PETG (Polyethylene Terephthalate Glycol-modified) for visibility.
- Break-resistant and lightweight, ensuring safe handling in lab environments.
- Non-treated surface for standard suspension cultures.
4. Sterile, Non-Pyrogenic & Contamination-Free
- Sterilized and DNase/RNase-free, making them ideal for biopharmaceutical applications.
- Non-pyrogenic and non-cytotoxic per ISO 10993-5 standards.
- Disposable single-use design eliminates cross-contamination risks.
5. Ergonomic & Convenient Handling
- Graduated markings allow quick volume assessment.
- Attached carrying handle for easy and safe transport.
- Peel-open packaging for fast and contamination-free access.
Specifications
| Property | Details |
|---|---|
| Brand | Thermo Scientific™ Nalgene™ |
| Capacity | 5 L |
| Material | PETG (Polyethylene Terephthalate Glycol-modified) |
| Bottom Shape | Angled (4115-5000) / Faceted (4115-5001) |
| Closure Material | HDPE (High-Density Polyethylene) |
| Closure Type | Vented |
| Closure Size | 96.5 mm |
| Outer Diameter | 214 mm |
| Height | 285 mm |
| Graduated | Yes |
| Sterility | Sterile |
| Autoclavable | No (Collapses when autoclaved) |
| Single-Use | Yes |
| Surface Treatment | Non-treated |
| Certifications | Non-pyrogenic, Non-cytotoxic (ISO 10993-5) |
| Unit Size | Case of 4 Flasks |
Available Variants & Ordering Information
| Catalog Number | Capacity (Metric) | Bottom Shape | Packaging |
|---|---|---|---|
| 4115-5000 | 5 L | Angled | Case of 4 |
| 4115-5001 | 5 L | Faceted | Case of 4 |
Applications
1. Cell Culture & Bioprocessing
- Suspension cell culture for CHO, HEK, and insect cells.
- Bioreactor pre-culture and seed expansion.
- Media preparation and optimization for high-yield protein production.
2. Transient Protein Expression & Viral Vector Production
- Optimized for ExpiCHO Expression System (Angled-Bottom Flask, 4115-5000).
- Supports large-scale protein and antibody expression.
- Used for viral vector production in gene therapy research.
3. Laboratory & Pharmaceutical Research
- Ideal for vaccine development and drug discovery.
- Sterile media storage and mixing.
- Culture expansion in regulated environments.
Storage & Handling
- Store in a cool, dry environment to maintain sterility.
- Do not autoclave; PETG material collapses under high heat.
- Use immediately after opening to prevent contamination.
The Thermo Scientific™ Nalgene™ 5-Liter Shake Flasks provide sterile, high-quality suspension cell culture solutions for biotechnology, pharmaceutical, and research applications. With two bottom designs (angled and faceted), robust PETG construction, and vented closures, these flasks offer optimum cell growth conditions and safe handling for high-yield production.
- Bottom shape: Angled bottom Faceted bottom