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Thermo Scientific™ Maxima Probe qPCR Master Mix (2X), with separate ROX vial
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Specifications:
| Application | qPCR, PCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Master Mix | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Maxima Probe qPCR Master Mix (2X), with separate ROX vial, is a high-performance, ready-to-use master mix designed for quantitative real-time PCR applications using probe-based detection systems. This formulation features Maxima Hot Start Taq DNA Polymerase and a proprietary buffer system for enhanced specificity, sensitivity, and reproducibility. The inclusion of dUTP enables optional carry-over prevention, and the separate ROX vial allows flexible adjustment of reference dye concentration to match various qPCR instruments.
Key Features and Benefits
- Flexible ROX Reference Dye – Supplied separately for optimal compatibility with different cyclers
- High Sensitivity and Specificity – Detects low-copy targets with minimal background
- Wide Dynamic Range – Accurate quantification across up to 9 logs
- Convenient Format – Ready-to-use 2X mix reduces setup time and error
- UDG Compatibility – dUTP included to support uracil DNA glycosylase-based contamination control
- Thermal Cycler Compatibility – Compatible with a broad range of qPCR platforms including Bio-Rad, Eppendorf, Roche, and Cepheid systems
Applications
- Gene Expression Analysis
- Pathogen Detection
- SNP Genotyping
- siRNA Validation
- Viral Load Quantification
- Microbial Load Testing
Specifications
| Property | Value |
|---|---|
| Format | 2X Master Mix with separate ROX vial |
| Polymerase | Maxima Hot Start Taq DNA Polymerase |
| Concentration | 2X |
| Detection Method | Probe-based (e.g., TaqMan®) |
| ROX Reference Dye | Supplied separately (50 μL of 50 µM ROX) |
| Sample Type | DNA, dsDNA |
| Reaction Volume | Standard 25 µL per reaction |
| Number of Reactions | 200 (K0261), 1,000 (K0262) |
| PCR Method | Real-Time PCR (qPCR) |
| Instrument Compatibility | Bio-Rad CFX96, Roche LightCycler 480, Eppendorf RealPlex, and others |
| GC-Rich PCR Performance | High |
| Storage | –20°C |
| Shipping Condition | Dry ice |
| Unit Size | Each |
Kit Contents
- 2 × 1.25 mL Maxima Probe qPCR Master Mix (2X)
- 50 µL ROX Solution (50 µM)
- 2 × 1.25 mL Nuclease-Free Water
The Maxima Probe qPCR Master Mix with separate ROX vial is an advanced solution for highly sensitive, specific, and reproducible real-time PCR. With flexible ROX control and compatibility across multiple platforms, it is ideal for research labs performing high-throughput and critical qPCR assays.
- Pack Size: 200 x 25 μL reactions. 1000 x 25 μL reactions