Thermo Scientific™ IPTG (isopropyl-β-D-thiogalactopyranoside) enzyme substrate dioxane-free, >99%, Ultrapure

Thermo Scientific IPTG is a dioxane-free, ultrapure (>99%) enzyme substrate used to induce expression of genes controlled by the lac operon. It functions as a stable, synthetic analog of lactose, inactivating the lac repressor and enabling transcription of genes downstream of the lac promoter. IPTG is widely used in molecular biology for gene expression studies and blue/white colony screening in conjunction with X-Gal.

Key Features:

1. High Purity and Stability:
   • >99% purity, ultrapure grade, free from dioxane.
   • Stable under freezer storage conditions.
2. Lac Operon Induction:
   • Inactivates the lac repressor to induce beta-galactosidase synthesis.
3. Application in Screening:
   • Used alongside X-Gal for blue/white colony screening to differentiate recombinant clones.
4. Versatile Applications:
   • Suitable for induction of cloned genes in bacterial systems such as E. coli.

Specifications:

Applications:

1. Gene Expression Induction:
   • Induces expression of genes controlled by the lac operon in bacterial systems.
2. Blue/White Screening:
   • Used in conjunction with X-Gal to identify recombinant clones in bacterial transformation experiments.
3. Protein Production:
   • Facilitates high-level expression of recombinant proteins in IPTG-inducible systems.

Advantages:

Handling and Storage:

• Storage Conditions: Store at freezer temperatures (-20°C or below).
• Ensure minimal exposure to moisture and air to maintain product stability.

This high-quality IPTG is ideal for all your gene expression and colony screening needs. Order now to enhance your molecular biology workflows!