Thermo Scientific™ Hypoxanthine 99.5%

Thermo Scientific™ Hypoxanthine is a high-purity crystalline powder (≥99.4% by HPLC) used as a critical nutrient additive in a wide range of cell culture systems and biochemical assays. As an essential purine derivative, hypoxanthine supports cell growth and is widely used in bacterial, parasite (e.g., Plasmodium falciparum), and animal cell cultures. It is particularly important in hybridoma technology as part of hypoxanthine-aminopterin-thymidine (HAT) selection media for the isolation of monoclonal antibodies.

In addition to its core role in purine metabolism, hypoxanthine has been utilized in vivo and in vitro studies, including enzyme kinetics, tRNA function, and post-mortem interval estimation through HPLC-UV analysis.

Key Features

• High Purity: HPLC-assayed purity ≥99.4%.
• Low Moisture Content: Loss on drying ≤0.5% for consistent storage and use.
• Low Ash Content: Sulfated ash ≤0.2% ensures minimal inorganic impurities.
• Validated by Infrared Spectrum: Confirms chemical identity and batch-to-batch consistency.
• Research-Grade Reagent: Suitable for biochemical, molecular biology, and cell culture applications.

Applications

• Cell Culture Supplementation:
  • Used in Plasmodium falciparum cultivation.
  • Nutrient additive in IMDM and hybridoma HAT selection media.
• Enzyme Kinetics:
  • Substrate for hypoxanthine-guanine phosphoribosyl transferase (HGPRT) assays.
• tRNA Research:
  • Involved as a 5'-base in anticodons of specific tRNA molecules.
• Animal Model Studies:
  • Evaluating xanthine oxidase activity in hyperuricemia mouse models.
• Forensic Biochemistry:
  • Standard for quantification of hypoxanthine in vitreous fluid (post-mortem interval estimation).

Specifications

Biochemical/Physiological Role

• Intermediate in Purine Metabolism: Precursor for nucleic acid biosynthesis.
• Functional Analogue of Guanine: Lacks an exocyclic amino group at position C(2), giving it distinct biochemical interactions.
• Supports Nucleotide Salvage Pathways: Especially important in cells with limited de novo purine synthesis.

Thermo Scientific™ Hypoxanthine, 99.5%, is a trusted, high-quality reagent essential for advanced molecular biology research, cell culture supplementation, and biochemical analysis. Its versatility across cell biology, parasitology, enzymology, and forensic applications makes it an indispensable component in life science laboratories.