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Thermo Scientific™ GeneJET Plant RNA Purification Kit
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Specifications:
| Application | RNA Extraction | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ GeneJET Plant RNA Purification Kit is designed for the rapid and efficient isolation of high-quality total RNA from a wide range of plant species and tissue types. Utilizing silica membrane spin column technology, the kit avoids phenol-chloroform extraction, resin-based methods, or alcohol precipitation, enabling fast and reproducible RNA purification.
The streamlined protocol takes less than 20 minutes after lysis, yielding up to 65 µg of high-integrity RNA from 50 mg of tissue. Purified RNA is suitable for all common downstream molecular biology applications, including RT-PCR, RT-qPCR, Northern blotting, microarray analysis, and next-generation sequencing (NGS).
Key Features
- Fast – RNA purification completed in 20 minutes after lysis.
- Convenient – protocol excludes clogging-prone filtration steps.
- High yield – up to 65 µg total RNA from 50 mg of fresh plant material.
- Versatile – compatible with leaves, roots, sprouts, and other plant tissues.
- High integrity – isolated RNA suitable for sensitive RNA-based analyses.
Applications
- RT-PCR and RT-qPCR
- cDNA library construction
- Microarray analysis
- Northern blotting and blot hybridization
- Nuclease protection assays
- In vitro translation
- Nucleic acid labeling
- Next-generation sequencing (NGS)
Technical Specifications
| Property | Value |
|---|---|
| Product Type | RNA Purification Kit |
| Isolation Technology | Silica membrane spin column |
| Sample Type | Plant tissues (leaves, roots, sprouts, etc.) |
| Scale | Mini-prep |
| Elution Volume | 50 µL |
| Typical Yield | Up to 65 µg RNA from 50 mg tissue |
| Purification Time | <20 min (after lysis) |
| RNA Size Range | Total RNA, high integrity |
| High-throughput Compatible | No (manual use) |
| Storage Conditions | Buffers at room temperature; columns at 2–8 °C |
| Shipping Condition | Room temperature |
Available Formats
| Catalog Number | Quantity | Format |
|---|---|---|
| K0801 | 50 preps | Spin column kit |
| K0802 | 250 preps | Spin column kit |
Kit Contents:
- Plant RNA Lysis Solution (200 mL)
- Wash Buffer WB 1 (200 mL, concentrated)
- Wash Buffer 2 (2 × 100 mL, concentrated)
- Nuclease-free water (125 mL)
- GeneJET RNA Spin Columns with collection tubes
- Collection tubes (2 mL and 1.5 mL)
Thermo Scientific™ GeneJET Plant RNA Purification Kit provides researchers with a fast, simple, and reliable method for isolating high-quality total RNA from diverse plant tissues. With high yields, integrity suitable for downstream analyses, and a time-efficient protocol, it is an essential tool for plant molecular biology, genomics, and transcriptomics research.
- Pack Size: 50 Preps 250 Preps