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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ FastDigest PstI is a high-performance restriction enzyme that recognizes the sequence 5′ CTGCA^G 3′ / 3′ G^ACGTC 5′, producing 3′ protruding ends. Optimized for rapid digestion, it achieves complete cleavage in 5–15 minutes at 37 °C using the universal FastDigest Buffer system.
As part of the FastDigest™ enzyme portfolio, PstI is 100% active in both the FastDigest and FastDigest Green Buffers, enabling single, double, or multiple digestions in one reaction without buffer changes or DNA clean-up steps. This streamlines cloning workflows and ensures compatibility with DNA-modifying enzymes such as T4 DNA Ligase, Klenow Fragment, T4 DNA Polymerase, and alkaline phosphatases.
Key Features
- Rapid digestion – complete DNA cleavage in 5–15 minutes.
- Universal buffer system – 100% enzyme activity in both FastDigest and FastDigest Green Buffers.
- No star activity – optimized buffer prevents nonspecific cleavage.
- Direct gel loading – FastDigest Green Buffer contains density reagent and tracking dyes.
- Seamless workflow – downstream modifying enzymes work directly in FastDigest Buffer.
- Broad selection – part of a portfolio of 176 FastDigest enzymes.
Applications
- Molecular cloning.
- Restriction site mapping.
- Genotyping and SNP analysis.
- Southern blotting.
- Restriction fragment length polymorphism (RFLP).
Technical Specifications
| Property | Value |
|---|---|
| Enzyme | PstI |
| Recognition Sequence | 5′ CTGCA^G 3′ / 3′ G^ACGTC 5′ |
| Cleavage Product | 3′ protruding ends |
| Isoschizomers | BspMAI |
| Optimal Reaction Temperature | 37 °C |
| Complete Digestion Time | 5–15 minutes |
| Compatible Buffers | 10X FastDigest Buffer, 10X FastDigest Green Buffer |
| Methylation Sensitivity | Not Dam-sensitive, Not Dcm-sensitive, Not CpG-sensitive |
| Heat Inactivation | No |
| Type IIS Restriction Enzyme | No |
| Research Category | Traditional cloning |
| Storage Temperature | –20 °C |
Available Formats
| Catalog Number | Quantity | Reactions | Format |
|---|---|---|---|
| FD0614 | 800 μL | ~800 reactions | Includes buffers |
| FD0615 | 5 × 500 μL (2,500 μL total) | ~2,500 reactions | Includes buffers |
Contents:
- FastDigest PstI enzyme
- 10X FastDigest Buffer (1.0 mL each)
- 10X FastDigest Green Buffer (1.0 mL each)
Thermo Scientific™ FastDigest PstI combines speed, precision, and convenience for molecular cloning and DNA analysis. Its universal buffer system, compatibility with downstream enzymes, and elimination of star activity streamline workflows while ensuring reproducibility. Offering rapid digestion in just minutes, it is a preferred choice for researchers requiring reliable DNA cleavage in cloning and genotyping applications.
- Pack Size: 800 μL (800 Reactions) 5 x 500 μL (2500 reactions)