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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ FastDigest NcoI is a rapid restriction enzyme that recognizes the sequence 5′ C^CATGG 3′ / 3′ GGTAC^C 5′, generating 5′ protruding ends. Optimized for performance at 37 °C, FastDigest NcoI enables complete DNA digestion in 5–15 minutes using the universal FastDigest Buffer system.
As part of the FastDigest™ enzyme collection, NcoI demonstrates 100% activity in both FastDigest and FastDigest Green Buffers. This eliminates the need for buffer changes when performing single, double, or multiple digestions and supports direct integration with downstream enzymatic processes such as ligation, fill-in, or dephosphorylation.
Key Features
- Fast digestion – complete cleavage within 5–15 minutes.
- Universal buffer system – all FastDigest enzymes are active in a single buffer.
- Direct loading – FastDigest Green Buffer includes density reagent and dyes for gel electrophoresis.
- No star activity – optimized buffer prevents nonspecific cleavage.
- Downstream compatibility – enzymes like T4 DNA Ligase, Klenow, and T4 DNA Polymerase are fully active in FastDigest Buffer.
- Comprehensive range – part of a portfolio of 176 FastDigest enzymes.
Applications
- Molecular cloning.
- Restriction site mapping.
- Genotyping.
- Southern blotting.
- Restriction fragment length polymorphism (RFLP).
- SNP analysis.
Technical Specifications
| Property | Value |
|---|---|
| Enzyme | NcoI |
| Recognition Sequence | 5′ C^CATGG 3′ / 3′ GGTAC^C 5′ |
| Cleavage Product | 5′ protruding ends |
| Isoschizomers | Bsp19I |
| Optimal Reaction Temperature | 37 °C |
| Digestion Time | 5–15 minutes |
| Compatible Buffers | 10X FastDigest Buffer, 10X FastDigest Green Buffer |
| Inactivation Temperature | 65 °C |
| Methylation Sensitivity | Not sensitive to Dam, Dcm, or CpG methylation |
| Heat Inactivation | Yes |
| Type IIS Restriction Enzyme | No |
| Research Category | Traditional cloning |
| Storage Temperature | –20 °C |
Available Formats
| Catalog Number | Quantity | Reactions | Format |
|---|---|---|---|
| FD0573 | 20 μL | ~20 reactions | Enzyme + Buffers |
| FD0574 | 100 μL | ~100 reactions | Enzyme + Buffers |
| FD0575 | 300 μL | ~300 reactions | Enzyme + Buffers |
Contents:
- FastDigest NcoI enzyme
- 10X FastDigest Buffer (1.0 mL)
- 10X FastDigest Green Buffer (1.0 mL)
Thermo Scientific™ FastDigest NcoI provides speed, precision, and workflow simplicity for molecular cloning and genetic analysis. Its universal buffer system, rapid digestion performance, and compatibility with downstream modifying enzymes make it a trusted choice for laboratories requiring reliable restriction digestion with high reproducibility and convenience.
- Pack Size: 300 μL (300 reactions) 20 μL (20 Reactions) 100 μL (100 Reactions)