Thermo Scientific™ EZ-Link™ Sulfo NHS-LC-LC-Biotin, No-Weigh™ Format

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-LC-Biotin is a long-spacer, water-soluble biotinylation reagent specifically designed for selective and efficient labeling of antibodies, proteins, and other primary amine-containing biomolecules. Featuring an extended 30.5 Å spacer arm and a sulfonated NHS ester for aqueous solubility, this reagent is especially effective for reducing steric hindrance in streptavidin-based binding assays.

The No-Weigh™ format provides the reagent in pre-weighed, single-use vials to preserve reagent stability and eliminate the need for precise weighing—improving consistency and reducing risk of contamination.

Key Features and Benefits

Extended Spacer Arm (30.5 Å)

• The longest in the EZ-Link™ Sulfo-NHS series.
• Minimizes steric hindrance, improving avidin/streptavidin accessibility and binding efficiency.

Amine-Reactive Chemistry

• Sulfo-NHS ester reacts efficiently with primary amines (e.g., lysine residues or N-terminal amines) to form irreversible amide bonds.

Water-Soluble & Cell-Impermeable

• Sulfonated group ensures complete solubility in aqueous buffers.
• Charged structure prevents passage through membranes, making it ideal for cell surface labeling.

Pre-Aliquoted No-Weigh™ Format

• Ready-to-use, pre-measured vials ensure accuracy and consistency.
• Reduces reagent degradation by avoiding repeated exposure to air and moisture.

Applications

• Biotinylation of antibodies and proteins for ELISA, flow cytometry, Western blotting, and pull-down assays
• Surface labeling of live or fixed cells without membrane permeation
• Affinity capture and purification of biotin-labeled proteins using streptavidin or avidin resins
• Optimization of bioconjugation experiments requiring longer spacer arms for enhanced accessibility

Technical Specifications

EZ-Link™ Sulfo-NHS-LC-LC-Biotin, No-Weigh™ Format combines high-performance biotinylation chemistry with practical convenience. Its extended spacer arm is ideal for minimizing steric effects in demanding detection and capture applications, while the pre-aliquoted format ensures reproducibility and stability. This reagent is perfectly suited for surface labeling and antibody conjugation in protein interaction and purification workflows.